產(chǎn)品名稱：Anti-Estrogen Related Receptor gamma抗體,雌激素受體相關(guān)蛋白3抗體

產(chǎn)品編號：BYK-6213R
英文名稱：Anti-Estrogen Related Receptor gamma
中文名稱：雌激素受體相關(guān)蛋白3抗體
英文別名：ERR 3; ERR G2; ERR gamma 2; ERR gamma-2; ERR3; ERR3_HUMAN; ERRG 2; ERRG2; ESRRG; Estrogen receptor related protein 3; Estrogen receptor-related protein 3; Estrogen-related receptor gamma; NR3B3; Nuclear receptor subfamily 3 group B member 3.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-Estrogen Related Receptor gamma抗體,雌激素受體相關(guān)蛋白3抗體

英文介紹：This gene encodes a member of the estrogen receptor-related receptor (ESRR) family, which belongs to the nuclear hormone receptor superfamily. All members of the ESRR family share an almost identical DNA binding domain, which is composed of two C4-type zinc finger motifs. The ESRR members are orphan nuclear receptors; they bind to the estrogen response element and steroidogenic factor 1 response element, and activate genes controlled by both response elements in the absence of any ligands. The ESRR family is closely related to the estrogen receptor (ER) family. They share target genes, co-regulators and promoters, and by targeting the same set of genes, the ESRRs seem to interfere with the ER-mediated estrogen response in various ways. It has been reported that the family member encoded by this gene functions as a transcriptional activator of DNA cytosine-5-methyltransferases 1 (Dnmt1) expression by direct binding to its response elements in the DNMT1 promoters, modulates cell proliferation and estrogen signaling in breast cancer, and negatively regulates bone morphogenetic protein 2-induced osteoblast differentiation and bone formation. Multiple alternatively spliced transcript variants have been identified, which mainly differ at the 5' end and some of which encode protein isoforms differing in the N-terminal region.
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Anti-Estrogen Related Receptor gamma抗體,雌激素受體相關(guān)蛋白3抗體Western Blotting 具體實(shí)驗(yàn)步驟：

Western，也稱Western blot、Western blotting、Western 印跡，是用抗體檢測蛋白的重要方法之一。
Western可以參考如下步驟進(jìn)行操作：
收集蛋白樣品(Protein sample preparation)
可以使用適當(dāng)?shù)牧呀庖毫呀赓N壁細(xì)胞、懸浮細(xì)胞或組織樣品。對于某些特定的亞細(xì)胞組份蛋白，例如細(xì)胞核蛋白、細(xì)胞漿蛋白、線粒體蛋白等，可以參考相關(guān)文獻(xiàn)提取這些亞細(xì)胞組份蛋白，也可以使用試劑盒進(jìn)行抽提。
收集完蛋白樣品后，為確保每個(gè)蛋白樣品的上樣量*，需要測定每個(gè)蛋白樣品的蛋白濃度。根據(jù)所使用的裂解液的不同，需要采用適當(dāng)?shù)牡鞍诐舛葴y定方法。因?yàn)椴煌牡鞍诐舛葴y定方法對于一些去垢劑和還原劑等的兼容性差別很大。

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