上海研盟生物科技有限公司Calcitonin抗體-降鈣素抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/p>
簡(jiǎn)單介紹:
中文名稱:降鈣素抗體
英文名稱:Anti-Calcitonin
產(chǎn)品編號(hào):BYK-2174R
產(chǎn)品別名:Alpha CGRP; alpha type CGRP; CALC 1; CALC A; CALC1; CALC1; CALCA; calcitonin 1; calcitonin gene related peptide 1; calcitonin gene related peptide I; Calcitonin related polypeptide alpha; CGRP 1; CGRP; CGRP I; CGRP1; CGRP1; CGRPI; CT; CT; Katacalcin; KC; KC; CALC_HUMAN; MGC126648; calcitonin isoform CT preproprotein.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實(shí)驗(yàn)
產(chǎn)品價(jià)格:請(qǐng)報(bào)價(jià)
說(shuō)明書:請(qǐng)?zhí)砑涌头苯铀魅?/p>
抗體來(lái)源:該指標(biāo)有兩種產(chǎn)品,一種是兔來(lái)源抗體,一種是鼠來(lái)源抗體
克隆類型:兔來(lái)源為多克隆抗體,鼠來(lái)源單克隆抗體
交叉反應(yīng):請(qǐng)索取說(shuō)明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲(chǔ) 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Calcitonin抗體-降鈣素抗體產(chǎn)品介紹:Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
多肽的溶解與保存
一、凍干多肽的溶解
1、多肽溶解的主要問(wèn)題是二級(jí)結(jié)構(gòu)的形成。雖然疏水肽鏈二級(jí)結(jié)構(gòu)的形成更明顯,但除zui短的肽鏈外,此現(xiàn)象產(chǎn)生在幾乎所有肽鏈,與極性無(wú)關(guān)。所以,溶解多肽的*個(gè)原則是使用無(wú)菌蒸餾水或去離子水,當(dāng)然無(wú)氧水。多肽溶液可能遇到細(xì)菌降解,為防止此情況的發(fā)生,應(yīng)溶解在無(wú)菌的蒸餾水中或用0.45或0.2孔徑的濾膜過(guò)濾除菌。含有Cys、Met、Trp的多肽很容易氧化,應(yīng)溶于無(wú)氧的水中,無(wú)氧水可通過(guò)注入惰性氣體(氮、氦、氬)減壓除氣得到;
2、若多肽不溶于純水,超聲處理有助于打碎顆粒并增加溶解度。注意:超聲處理會(huì)引起溶液發(fā)熱或多肽降解;
3、若多肽含有多個(gè)堿性氨基酸,使用(1-10%)的乙酸水溶液:對(duì)于疏水性強(qiáng)的多肽,應(yīng)使用50%的乙酸;
4、若多肽中含有大量酸性氨基酸,可用氨溶液(1-10%)或乙酸乙基*或重碳酸鹽等揮發(fā)性的堿性緩沖液溶解。pH值在層析前必須調(diào)整;
5、異丙醇和乙腈能溶解中等大小的多肽。若肽要上柱,有機(jī)溶劑的量必須很少否則將嚴(yán)重影響停留時(shí)間;
6、若多肽因含有Val、Leu、Met、Phe、Tyr、Ala等芳香鏈而高度疏水、或?yàn)橹行噪臅r(shí),DMF或DMSO等膜變性劑的使用,有利于多肽的溶解:
a.高濃度模變性劑通過(guò)破壞多肽的二級(jí)結(jié)構(gòu)而助溶;
b.膜變性劑適于多肽分析液的制備,但可能會(huì)對(duì)其生物活性的研究工作造成干擾;
c.DMF是變性劑(zui高濃度可達(dá)30%),滴加至多肽溶解;
d.反相層析法時(shí),DMF將與洗脫液前鋒一起流出,根據(jù)入量的多少,峰值可能很高。大多數(shù)肽能在大量DMF流出后幾分鐘內(nèi)流出,若肽鏈很小,洗脫太早,多肽的量將很低。
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