★研盟生物 ★合作： ★客服
相關(guān)標(biāo)記抗體：HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體
上海研盟生物科技有限公司Anti-Minibrain抗體*，主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送，您也可以直接我司在線客服索取。客服
英文名稱：Anti-Minibrain antibody
中文名稱：Minibrain 抗體
Minibrain 抗體保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
★研盟生物★Minibrain 抗體,Anti-Minibrain抗體
石蠟切片的免疫組化SP法參考步驟：
1.60℃烤片2小時(shí)
2.脫蠟。二甲苯脫蠟15分鐘，三次
3.水化。依次經(jīng)過無水乙醇、95%、90%、80%、70%，各5分鐘，蒸餾水（或自來水）5分鐘
4.PBS洗5分鐘，三次
5.抗原修復(fù)。枸櫞酸緩沖液（約92-95℃）煮沸（沸水浴）修復(fù)15分鐘，自然涼至室溫。PBS洗5分鐘，三次
6.3%雙氧水消化內(nèi)源性的過氧化物酶，20分鐘，PBS洗5分鐘，三次
7.正常山羊血清封閉，37℃20分鐘
8.一抗孵育，4℃過ye.
9.PBS洗5分鐘，三次
10.二抗孵育，37℃20分鐘（二抗為*標(biāo)記的羊抗兔IgG），PBS洗5分鐘，三次
11.三抗孵育，37℃20分鐘（三抗為辣根酶標(biāo)記的鏈霉親和素），PBS洗5分鐘，三次
12.DAB顯色，鏡下觀察結(jié)果，適時(shí)終止
13.蘇木素復(fù)染5分鐘，自來水沖洗片刻，1%的鹽酸酒精（75%）溶液（請(qǐng)自行配置，配制比例為：100ml 75%酒精+1ml濃鹽酸）分化30秒，自來水沖洗5分鐘藍(lán)化
14.脫水。依次經(jīng)過70%、80%、90%、95%、無水乙醇，各5分鐘，二甲苯15分鐘，三次
15.封片，中性樹膠封片
Minibrain 抗體,Anti-Minibrain抗體溶解方法:
方法1：我們的抗體（凍干粉）已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑（疊氮鈉或*），您只需要加入滅菌的雙蒸水就行了。抗體溶解后，置于-20℃保存，分裝后使用，避免反復(fù)凍融，可保證至少1年有效；
方法2：也可以只加入一半體積的雙蒸水，再用一半體積的甘油補(bǔ)足，置于-20℃保存，這樣抗體不會(huì)凍，有利于抗體的穩(wěn)定。
后續(xù)試驗(yàn)時(shí)，再使用對(duì)應(yīng)的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個(gè)月有效；若在-20℃下保存（*），至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
簡(jiǎn)單介紹：
產(chǎn)品別名：MNB_DROME; Serine/threonine-protein kinase minibrain; mnb; Dmel_CG42273; Dmel_CG7826; Dmel_CG7835; CG42273; CG7826; CG7835; Dm1; Dmel\CG42273; Dyrk1; DYRK1; ME-IV; min; Mnb; MNB.
抗體來源：Rabbit or Mouse
保質(zhì)期:1年
克隆類型：Polyclonal or monoclonal
性 狀：Lyophilized or Liquid
濃 度：1mg/1ml
背景介紹：This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms.
相關(guān)優(yōu)質(zhì)產(chǎn)品：
polyprotein(duck hepatitis A virus, DHAV) 鴨甲型肝炎A病毒抗體
Des(Desmin) 結(jié)蛋白抗體
Digoxin（Dig-BSA） 兔抗地gao辛抗體
Digoxin(Mouse Digoxin Monoclonal) 小鼠抗地gao辛單克隆抗體
DISC1（disrupted in schizophrenia1）(CT) DISC1 C端抗體
DISC1（disrupted in schizophrenia1）（NT） DISC1 N端抗體
DLK1(Delta like 1 homolog) 穿膜蛋白DLK1抗體
DLX4 (Dsital-less homebox4 isoformα/β) 兔抗DLX4抗體
DKK1(Dickkopf 1) 抑癌蛋白DKK1抗體
DMBT1 (Deleted in malignant brain tumor 1) 抑癌基因抗體
Dnmt1(DNA metYMltransferase 1) DNA甲基轉(zhuǎn)移酶1抗體
Dnmt3a(cytosine-5)-metYMltransferase 3A) DNA甲基轉(zhuǎn)移酶-3α抗體
Dnmt3β (DNA (cytosine-5)-metYMltransferase 3 β) DNA甲基轉(zhuǎn)移酶-3β抗體
DNA-PKcs(DNA-dependent protein kinase catalytic subunit) DNA依賴蛋白激酶催化亞基抗體
DOK-1（downstream of tyrosine kinase 1） *激酶衰減蛋白/Ras GTP酶活化蛋白抗體
DPPA2 (developmental pluripotency associated gene2) 多能發(fā)育相關(guān)基因2抗體
DR3/APO3/TNF-αR/TRAMP(death receptor 3) 抗APO-3/DR3死亡受體3抗體
DR4/APO2/TRAILR1 (Death receptor 4) 死亡受體4抗體
DR5/TNFBYK10B/CD262 (Death receptor 5) 腫瘤細(xì)胞調(diào)亡素/死亡受體5抗體
