細胞名稱：人卵巢畸胎瘤細胞；PA-1培養(yǎng)
細胞形態(tài)： 細胞株
生長特性：貼壁 懸浮 半貼壁
傳代時間：2-3天 3-5天
傳代比例：1:2~1:3 1:1~1:2
儲存：液氮
保存與運輸： 干冰常溫運輸
細胞在培養(yǎng)瓶長成致密單層后，已基本上飽和，為使細胞能繼續(xù)生長，同時
也將細胞數(shù)量擴大，就必須進行傳代(再培養(yǎng))。 傳代培養(yǎng)也是一種將細胞種保存下去的方法。同時也是利用培養(yǎng)細胞進行各種實驗的必經(jīng)過程。懸浮型細胞直接分瓶就可以，而貼壁細胞需經(jīng)消化后才能分瓶。
人卵巢畸胎瘤細胞；PA-1培養(yǎng)細胞培養(yǎng)
1、冷凍管應如何解凍？
取出冷凍管后， 須立即放入37 ℃水槽中快速解凍， 輕搖冷凍管使其在1 分鐘內(nèi)全部融化， 并注意水面不可超過冷凍管蓋沿， 否則易發(fā)生污染情形。另冷凍管由液氮桶中取出解凍時， 必須注意安全， 預防人卵巢畸胎瘤細胞；PA-1培養(yǎng)冷凍管之爆裂。
2、細胞冷凍管解凍培養(yǎng)時， 是否應馬上去除DMSO？
除少數(shù)特別注明對DMSO 敏感之細胞外， 絕大部分細胞株（包括懸浮性細胞）， 在解凍之后， 應直接放入含有10-15ml新鮮培養(yǎng)基之培養(yǎng)角瓶中， 待隔天再置換新鮮培養(yǎng)基以去除DMSO 即可， 如此可避免大部分解凍后細胞無法生長或貼附之問題。
3、可否使用與原先培養(yǎng)條件不同之培養(yǎng)基？
不能。每一細胞株均有其特定使用且已適應之細胞培養(yǎng)基， 若驟然使用和原先提供之培養(yǎng)條件不同之培養(yǎng)基， 細胞大都無法立即適應， 造成細胞無法存活。
4、可否使用與原先培養(yǎng)條件不同之血清種類？
不能。血清是細胞培養(yǎng)上一個極為重要的營養(yǎng)來源， 所以血清的種類和品質(zhì)對于細胞的生長會產(chǎn)生*的影響。來自不同物種的血清， 在一些物質(zhì)或分子的量或內(nèi)容物上都有所不同，血清使用錯誤常會造成細胞無法存活。
人卵巢畸胎瘤細胞；PA-1培養(yǎng)注意事項：
1. 收到細胞后首先觀察細胞瓶是否完好，培養(yǎng)液是否有漏液、渾濁等現(xiàn)象，若有上述現(xiàn)象發(fā)生請及時和我們。
2. 仔細閱讀細胞說明書，了解細胞相關(guān)信息，如細胞形態(tài)、所用培養(yǎng)基、血清比例、所需細胞因子等。
3. 用75%酒精擦拭細胞瓶表面，顯微鏡下觀察細胞狀態(tài)。因運輸問題貼壁細胞會有少量從瓶壁脫落，將細胞置于培養(yǎng)箱內(nèi)靜置培養(yǎng)，隔天再取出觀察。此時多數(shù)細胞均會貼壁，若細胞仍不能貼壁請用臺盼藍染色測定細胞活力，如果證實細胞活力正常，請將細胞離心后用新鮮培養(yǎng)基再次貼壁培養(yǎng)；如果染色結(jié)果顯示細胞無活力，請拍下照片及時和我們，信息確認后我們?yōu)槟倜赓M寄送一次。
4. 請客戶用相同條件的培養(yǎng)基用于細胞培養(yǎng)。培養(yǎng)瓶內(nèi)多余的培養(yǎng)基可收集備用，細胞傳代時可以一定比例和客戶自備的培養(yǎng)基混合，使細胞逐漸適應培養(yǎng)條件。
5. 建議客戶收到細胞后前3天各拍幾張細胞照片，記錄細胞狀態(tài)，便于和公司技術(shù)部溝通交流。
6. 該細胞只能用于科研，不得用于臨床應用?！加猛尽剑荷芯?。沉淀鉀、銣、銫、銨，以與鈉分離。

（石蠟切片需做抗原修復）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user. 
保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 GNL2 is a nucleolar guanasine-triphosphate binding protein that is ubiquitously expressed at low levels in almost all tissues. GNL2 is involved in the crucial process of trafficking proteins out of the nucleus. Specifically, it is a GTPase that interacts with the 60s preribosomal subunit in the nucleus and facilitates export of the subunit into the cytoplasm. GTPases are responsible for the hydrolysis of GTP by way of a protein region dubbed the G domain. GTPases are often involved in the translocating proteins through membranes gleaning energy for the activity by hydrolizing GTP. GNL2 shares G domain homology and some functionality with nucleostemin (GNL3), another nuclear GTPase. Highest expression of GNL2 is found in testis.
Function : GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.
Subcellular Location : Nucleus; nucleolus.
Tissue Specificity : Ubiquitously expressed at relatively low levels in all human tissues tested, with the highest level of expression in the testes.
Similarity : Belongs to the MMR1/HSR1 GTP-binding protein family. NOG2 subfamily.
Contains 1 G (guanine nucleotide-binding) domain.
Database links : UniProtKB/Swiss-Prot: Q13823.1

英文名稱  Anti-GNL3L
中文名稱  *核苷酸結(jié)合蛋白3樣蛋白抗體
別    名  GNL3L_HUMAN; FLJ10613; Guanine nucleotide binding protein-like 3 (nucleolar)-like; novel GTPase; OTTHUMP00000023374.
濃    度  1mg/1ml
規(guī) 格  0.2ml/200μg    
抗體來源  Rabbit 
克隆類型  polyclonal
交叉反應  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit
產(chǎn)品類型  一抗   
研究領(lǐng)域  細胞生物 免疫學 染色質(zhì)和核信號 G蛋白偶聯(lián)受體 G蛋白信號 
蛋白分子量  predicted molecular weight: 66kDa
性    狀  Lyophilized or Liquid
免 疫 原  KLH conjugated synthetic peptide derived from human GNL3L
亞    型  IgG
純化方法  affinity purified by Protein A
儲 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500
（石蠟切片需做抗原修復）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user. 
保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 GTPases from the MMR1/HSR1 GTP-binding protein subfamily are circularly rearranged G-motifs that play a critical role in maintaining normal cell growth. Deletion of these genes results in severe growth defects with a marked reduction in mature rRNA species and a concomitant accumulation of the 35S pre-rRNA transcript. Deletion also causes the ribosomal protein Rpl25a to fail exportation from the nucleolus. Deletion of any of the G-domain motifs will result in a null phenotype and nuclear/nucleolar localization that lacks the nucleolar export of preribosomes and is accompanied by a distortion of the nucleolar structure. GNL3L (guanine nucleotide binding protein-like 3 (nucleolar)-like) is a 582 amino acid nuclear protein that belongs to the MMR1/HSR1 GTP-binding protein family. Containing one G (guanine nucleotide-binding) domain, GNL3L is required for normal processing of ribosomal pre-rRNA and for cell proliferation.
Function : Stabilizes TERF1 omeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP.
Subunit : Interacts with MDM2; this interaction, which occurs in the nucleoplasm, stabilizes MDM2. Indirectly interacts with TP53, via MDM2-binding. Interacts with TERF1; this interaction probably occurs in the nucleoplasm and is increased during mitosis, when the nucleolus is disassembled. This binding may promote TERF1 homodimerization. Interacts with TERT.
Subcellular Location : Nucleus, nucleolus.
Similarity : Belongs to the MMR1/HSR1 GTP-binding protein family.
Contains 1 G (guanine nucleotide-binding) domain.
Database links : UniProtKB/Swiss-Prot: Q9NVN8.1

英文名稱  Anti-GNPTAB
中文名稱  溶酶體累積病相關(guān)蛋白/口吃相關(guān)蛋白抗體
別    名  N-acetylglucosamine-1-phosphotransferase subunit beta; EC=2.7.8.17; GlcNAc-1-phosphotransferase subunits alpha/beta; GNPTA; GNPTA_HUMAN; Gnptab; KIAA1208; Stealth protein GNPTAB; UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta.
濃    度  1mg/1ml
規(guī) 格  0.2ml/200μg    
抗體來源  Rabbit 
克隆類型  polyclonal
交叉反應  Human, Mouse, Rat, Dog, Pig, Cow, Sheep
產(chǎn)品類型  一抗   
研究領(lǐng)域  腫瘤 細胞生物 信號轉(zhuǎn)導 新陳代謝 
蛋白分子量  predicted molecular weight: 39kDa
性    狀  Lyophilized or Liquid
免 疫 原  KLH conjugated synthetic peptide derived from human N-acetylglucosamine-1-phosphotransferase subunit beta
亞    型  IgG
純化方法  affinity purified by Protein A
儲 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4