表皮生長因子試劑盒_EGF酶聯(lián)免疫分析
齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性,在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。
表皮生長因子試劑盒_EGF酶聯(lián)免疫分析操作步驟(僅供參考具體詳見說明書)
實驗開始前,請?zhí)崆芭渲煤盟性噭?,試劑或樣品稀釋時,均需混勻,混勻時盡量避免起泡。每次檢測都應該做標準曲線。如樣品濃度過高時,用樣品稀釋液進行稀釋,以使樣品符合試劑盒的檢測范圍。
1.加樣:分別設空白孔、標準孔、待測樣品孔??瞻卓准訕悠废♂屢?00μl,余孔分別加標準品或待測樣品100μl,注意不要有氣泡,加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻,酶標板加上蓋或覆膜,37℃反應120分鐘。
為保證實驗結果有效性,每次實驗請使用新的標準品溶液。
2. 棄去液體,甩干,不用洗滌。每孔加*標記抗體工作液 100μl(取1μl*標記抗體加99μl*標記抗體稀釋液的比例配制,輕輕混勻,在使用前一小時內配制),37℃,60分鐘。
3.溫育60分鐘后,棄去孔內液體,甩干,洗板3次,每次浸泡1-2分鐘,350μl/每孔,甩干。
4.每孔加辣根過氧化物酶標記親和素工作液(同*標記抗體工作液) 100μl,37℃,60分鐘。
5.溫育60分鐘后,棄去孔內液體,甩干,洗板5次,每次浸泡1-2分鐘,350μl/每孔,甩干。
6.依序每孔加底物溶液90μl,37℃避光顯色(30分鐘內,此時肉眼可見標準品的前3-4孔有明顯的梯度藍色,后3-4孔梯度不明顯,即可終止)。
7.依序每孔加終止溶液50μl,終止反應(此時藍色立轉黃色)。終止液的加入順序應盡量與底物液的加入順序相同。為了保證實驗結果的準確性,底物反應時間到后應盡快加入終止液。
8.用酶聯(lián)儀在450nm波長依序測量各孔的光密度(OD值)。 在加終止液后15分鐘以內進行檢測。
注:
1. 用戶在初次使用試劑盒時,應將各種試劑管離心數(shù)分鐘,以便試劑集中到管底。
2. 每次實驗留一孔作為空白調零孔,該孔不加任何試劑,只是zui后加底物溶液及2N H2SO4。測量時先用此孔調OD值至零。
3. 為防止樣品蒸發(fā),試驗時將反應板放于鋪有濕布的密閉盒內,酶標板加上蓋或覆膜。
4. 未使用完的酶標板或者試劑,請于2-8℃保存。標準品、*標記抗體工作液、辣根過氧化物酶標記親和素工作液請依據(jù)所需的量配置使用。請勿重復使用已稀釋過的標準品、*標記抗體工作液或、辣根過氧化物酶標記親和素工作液。
5. 建議檢測樣品時均設雙孔測定,以保證檢測結果的準確性。
表皮生長因子試劑盒_EGF酶聯(lián)免疫分析試劑盒安全性提示:
1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2.實驗中不要吃喝、抽煙或使用化妝品。
3.不要用嘴吸取試劑盒里的任何成份
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齊一生科技(上海)有限公司 感謝您的查閱與咨詢!期待與您的真誠合作!QY-H10766 Human anti-liver cell membrane antibody,LMA ELISA Kit 人抗肝細胞膜抗體(LMA)ELISA酶法試劑盒
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QY-H10774 Human crystal proteins β,Cryβ ELISA Kit 人晶體蛋白β(Cryβ)ELISA酶法試劑盒
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Phospho-Tuberin/TSC2 (Ser1254) 磷酸化馬鈴薯球蛋白(結節(jié)性硬化)抗體
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Phospho-Tyrosine Hydroxylase (Ser31) 磷酸化*羥化酶抗體
Phospho-VASP (Ser157) 磷酸化血管擴張刺激磷蛋白抗體
CaMK2a(calcium/calmodulin-dependent protein kinase II alpha) 鈣/鈣調素依賴蛋白激酶2α抗體
phospho-CaMK2a(pThr286) 磷酸化鈣/鈣調素依賴蛋白激酶2α抗體
Camk1g(Calcium/calmodulin dependent protein kinase IG) 鈣/鈣調蛋白依賴蛋白激酶IG抗體
CaMK2b(calcium/calmodulin-dependent protein kinase II beita) 鈣/鈣調素依賴蛋白激酶2b抗體
CAP1/Park7/DJ-1 Park7/DJ-1/CAP1抗體
CAP1/Park7/DJ-1 Park7/DJ-1/CAP1抗體
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QY229-1 碳酸銀 Silver carbonate 534-16-7 CP,98% "儲存條件: RT,避光
介紹:
新沉淀時為亮黃色,干燥后變?yōu)閹Щ尹S色,在光線下色澤暗,久置色也變暗,感光性*。約在220℃時分解為氧化銀和二氧化碳,更高溫度時分解為金屬銀。水,易溶于稀硝酸、乙酸、*溶液、氟化鉀、氨水和氰化堿溶液,不溶于冷水和醇。有刺激性。新制得的為淺黃色粉未。溶于氨水、稀硝酸、,不溶于水和醇。避光保存。"
QY230-1 碘化銀 Silver iodide 7783-96-2 CP "熔點: 557°C
沸點: 1506°C
比重: 5·68
儲存條件: RT,避光
溶解性: 易溶于氰化堿和碘化堿溶液,溶于濃的氫碘酸、溴化堿、氯化堿、硫氰酸堿、硫代硫酸堿、*溶液,幾乎不溶于水、氫碘酸以外的酸和碳酸銨溶液
用途: 微量分析銫。人工降雨。攝影。制藥。
敏感性: 對光敏感
介紹: 無氣味。遇空氣與光逐漸變?yōu)楹谏D芫徛嘏c沸的濃酸反應,而不與熱的氫氧化堿溶液反應。"
QY231-1 硫酸銀 Silver sulfate 10294-26-5 AR,99.7% "熔點: 652°C
沸點: 1085°C
比重: 5·45
儲存條件: RT,避光
溶解性: 能溶于硝酸、氨水及濃硫酸和熱水,,不溶于乙醇。能慢慢地溶于150分水和71分沸水。
用途: 比色測定硝酸鹽、磷酸鹽和氟,測定乙烯,水質分析中鉻和鈷的測定。
敏感性: 對光線敏感
介紹:
在光線下逐漸變黑色。在1085度分解。"
QY232-1 乙酸銀 Silver acetate 563-63-3 CP,99% "儲存條件: RT,避光
溶解性: 易溶于稀硝酸,溶于100份冷水、35份沸水。
敏感性: 對光敏感
介紹: 對光敏感。有刺激性"
QY233-1 氧化金 Gold oxide 1303-58-8 BR,89% "比重: 7·483
儲存條件: RT
敏感性: 對光敏感
介紹: 不溶于水,溶于HCl、HNO3、NaCN溶液中"
QY234-1 2,5-二苯惡唑 PPO 92-71-7 BR,99% "熔點: 72°C
沸點: 360°C
比重: 1·06
儲存條件: RT,避光
溶解性: 溶于醇、醚和石油醚,不溶于水。
用途: 該品是閃爍試劑,用于閃爍光譜。
介紹: 能隨水蒸氣微揮發(fā)。"
QY235-1 1,4-雙(5-苯基-2-惡唑)苯 POPOP 1806-34-4 AR "熔點: 242-246
儲存條件: RT
溶解性: 不溶于水、乙醇,微溶于甲苯,"
QY236-1 咪唑 Imidazole 288-32-4 AR,99%,腐蝕品 "熔點: 90-91°C
沸點: 257°C
比重: 1·0975
儲存條件: RT"
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PIK3CD Antibody O00329 50ug/50ul ,100ug/100ul Human WB IF/ICC
CITED2 Antibody Q99967 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
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NEUROG2 Antibody Q9H2A3 50ug/50ul ,100ug/100ul Human WB
PKMYT1 Antibody Q99640 50ug/50ul ,100ug/100ul Human WB
KCNJ2 Antibody P63252 50ug/50ul ,100ug/100ul Human WB
FDPS Antibody P14324 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
YY1 Antibody P25490 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
PLK4 Antibody O00444 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
FDFT1 Antibody P37268 50ug/50ul ,100ug/100ul Human WB
ARF1 Antibody P84077 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
GOPC Antibody Q9HD26 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
FOXA1 Antibody P55317 50ug/50ul ,100ug/100ul Human WB
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MT-CO2 Antibody P00403 50ug/50ul ,100ug/100ul Human WB
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PLA2G1B Antibody P04054 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
SLC4A1 Antibody P02730 50ug/50ul ,100ug/100ul Human WB
CDC6 Antibody Q99741 50ug/50ul ,100ug/100ul Human WB
FLII Antibody Q13045 50ug/50ul ,100ug/100ul Human WB
DAZL Antibody Q92904 50ug/50ul ,100ug/100ul Human WB
MLLT11 Antibody Q13015 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
CD48 Antibody P09326 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
URM1 Antibody Q9BTM9 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
ATG3 Antibody Q9NT62 50ug/50ul ,100ug/100ul Human WB
NODAL Antibody Q96S42 50ug/50ul ,100ug/100ul Human, Mouse WB
FDX1 Antibody P10109 50ug/50ul ,100ug/100ul Human, Mouse WB
ITCH Antibody Q96J02 50ug/50ul ,100ug/100ul Human WB
NUDC Antibody Q9Y266 50ug/50ul ,100ug/100ul Human, Mouse WB
UBE2N Antibody P61088 50ug/50ul ,100ug/100ul Human, Mouse WB
USP28 Antibody Q96RU2 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
HDLBP Antibody Q00341 50ug/50ul ,100ug/100ul Human WB IHC
ITCH Antibody Q96J02 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
S100A2 Antibody P29034 50ug/50ul ,100ug/100ul Human WB IHC
SSTR5 Antibody P35346 50ug/50ul ,100ug/100ul Human WB
TSPO Antibody P30536 50ug/50ul ,100ug/100ul Human WB
PAM Antibody P19021 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
ACTC1 Antibody P68032 50ug/50ul ,100ug/100ul Human, Mouse WB
STXBP1 Antibody P61764 50ug/50ul ,100ug/100ul Human WB
SLC4A1 Antibody P02730 50ug/50ul ,100ug/100ul Human WB
GFPT1 Antibody Q06210 50ug/50ul ,100ug/100ul Human,Rat WB
ABCA1 Antibody O95477 50ug/50ul ,100ug/100ul Human WB
RIPK1 Antibody Q13546 50ug/50ul ,100ug/100ul Human, Mouse WB
CD163 Antibody Q86VB7 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
MSLN Antibody Q13421 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
GBA Antibody P04062 50ug/50ul ,100ug/100ul Human, Mouse WB
DUSP3 Antibody P51452 50ug/50ul ,100ug/100ul Human, Mouse WB IHC
COPS8 Antibody Q99627 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
ING2 Antibody Q9H160 50ug/50ul ,100ug/100ul Human WB
TRAM2 Antibody Q15035 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
ATG3 Antibody Q9NT62 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
UBE2M Antibody P61081 50ug/50ul ,100ug/100ul Human,Rat WB IHC
SSR1 Antibody P43307 50ug/50ul ,100ug/100ul Human WB
TLN2 Antibody Q9Y4G6 50ug/50ul ,100ug/100ul Human WB
CTSS Antibody P25774 50ug/50ul ,100ug/100ul Human WB
HTT Antibody P42858 50ug/50ul ,100ug/100ul Human WB
EEA1 Antibody Q15075 50ug/50ul ,100ug/100ul Human WB
OS9 Antibody Q13438 50ug/50ul ,100ug/100ul Human WB IHC
BCAM Antibody P50895 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
GRK1 Antibody Q15835 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
TIMELESS Antibody Q9UNS1 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
CD97 Antibody P48960 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
UNC13D Antibody Q70J99 50ug/50ul ,100ug/100ul Human, Mouse WB
NR2C2 Antibody P49116 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
CDH10 Antibody Q9Y6N8 50ug/50ul ,100ug/100ul Human WB
PPP1R9A Antibody Q9ULJ8 50ug/50ul ,100ug/100ul Human WB
SEPP1 Antibody P49908 50ug/50ul ,100ug/100ul Human WB IHC
NEK6 Antibody Q9HC98 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
PNP Antibody P00491 50ug/50ul ,100ug/100ul Human WB IHC
MAPKAPK2 Antibody P49137 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
LGALS8 Antibody O00214 50ug/50ul ,100ug/100ul Human, Mouse WB
CD53 Antibody P19397 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
UIMC1 Antibody Q96RL1 50ug/50ul ,100ug/100ul Human WB
F5 Antibody P12259 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
KIF3A Antibody Q9Y496 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
CD82 Antibody P27701 50ug/50ul ,100ug/100ul Human WB
PSMA5 Antibody P28066 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
JAG1 Antibody P78504 50ug/50ul ,100ug/100ul Human WB
IFITM3 Antibody Q01628 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
亞麻酸含量測定試劑盒 高效液相色譜法 100T 咨詢
*含量測定試劑盒 高效液相色譜法 100T 咨詢
*含量測定試劑盒 高效液相色譜法 100T 咨詢
*含量測定試劑盒 高效液相色譜法 100T 咨詢
*3含量測定試劑盒 高效液相色譜法 100T 咨詢
*1含量測定試劑盒 高效液相色譜法 100T 咨詢
*含量測定試劑盒 高效液相色譜法 100T 咨詢
*1含量測定試劑盒 高效液相色譜法 100T 咨詢
*2含量測定試劑盒 高效液相色譜法 100T 咨詢
鹽酸水蘇堿 Stachydrine hydrochloride 4136-37-2 20mg
延齡草苷 Diosgenin glucoside 14144-06-0 20mg
原阿片堿 Protopine 130-86-9 20mg
* Etoposide 33419-42-0 20mg
鹽酸益母草堿 Leonurine hydrochloride 24697-74-3 20mg
乙酰紫堇靈 Acetylcorynoline 18797-80-3 20mg
紫堇靈 (+)-corynoline 18797-79-0 20mg
脫氫紫堇堿 Dehydrocorydaline 30045-16-0 20mg
異鼠李素--3-O-β-D-葡萄糖苷 Isorhamnetin-3-O-β-D-Glucoside 5041-82-7 20mg
鴨腳樹葉堿 2α,5α-Epoxy-1,2-dihydroakuammilan-17-oic acid methyl ester 4684-32-6 20mg
銀鍛苷 Tiliroside 20316-62-5 20mg
3-乙?;?β乳香酸 3-Acetyl-β-boswellic acid 5968-70-7 10mg
3-乙酰基-11-酮基乳香酸 beta-boswellic acid,3-acetyl,11-keto 20mg
QYS-23066 尿酸含量試劑盒 微量法
QYS-23067 尿酸含量試劑盒 可見分光光度法
QYS-23068 硫氧還蛋白過氧化物酶(TPX)試劑盒 微量法
QYS-23069 硫氧還蛋白過氧化物酶(TPX)試劑盒 紫外分光光度法
QYS-23070 *過氧化物酶(GPX)試劑盒 微量法
QYS-23071 *過氧化物酶(GPX)試劑盒 GR法
QYS-23072 *S-轉移酶(GST)試劑盒 微量法
QYS-23073 *S-轉移酶(GST)試劑盒 紫外分光光度法
QYS-23074 抗壞血酸(AsA)/*含量試劑盒 微量法
QYS-23075 抗壞血酸(AsA)/*含量試劑盒 紫外分光光度法
QYS-23076 抗壞血酸(AsA)/*含量試劑盒 高效液相色譜法
QYS-23077 抗壞血酸(AsA)/*含量試劑盒 滴定法
QYS-23078 總巰基試劑盒 微量法
QYS-23079 總巰基試劑盒 可見分光光度法
QYS-23080 植物花色苷試劑盒 微量法
QYS-23081 植物花色苷試劑盒 可見分光光度法
QYS-23082 單寧含量試劑盒 微量法
QYS-23083 單寧含量試劑盒 可見分光光度法
QYS-23084 超氧陰離子清除能力試劑盒 微量法
QYS-23085 超氧陰離子清除能力試劑盒 可見分光光度法
QYS-23086 非蛋白質巰基試劑盒 微量法
表皮生長因子(EGF)試劑盒 96次/48次 血清、血漿、組織等