詳細(xì)介紹
概述
Sepax SEC鍵合固定相是采用的表面修飾技術(shù)，通過在高純度具有良好機械穩(wěn)定性的硅膠基質(zhì)上，鍵合一層均勻的納米厚度的中性親水涂層而制備得到。由于采用可控的化學(xué)修飾技術(shù)，因此能確保柱與柱之間有著可靠的重現(xiàn)性。SEC填料采用化學(xué)鍵合技術(shù)，表面親水涂層覆蓋*，因此不僅具有優(yōu)異的穩(wěn)定性，而且對蛋白等生物樣品的非特異性吸附作用也非常小。精心設(shè)計的大孔體積可保證高的分離容量以及優(yōu)異的分辨率。廣泛應(yīng)用于生物分子及水溶性聚合物的分離和檢測。
應(yīng)用領(lǐng)域
蛋白藥物 （*、免疫球蛋白、HGH、EPO等）
多肽(Peptide)
水溶性多聚物（多糖、PEG）
抗生素（頭孢類抗生素）
抗體（Antibodies）、疫苗（Vaccine）
病毒蛋白(Virus Protein)、細(xì)胞發(fā)酵液、DNA
技術(shù)參數(shù)
| 固定相 | SRT SEC-100 | SRT SEC-150 | SRT SEC-300 | SRT SEC-500 | SRT SEC-1000 | SRT SEC-2000 |
| 材料 | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica |
| 顆粒大小 | 5 mm | 5 mm | 5 mm | 5 mm | 5 mm | 5 mm |
| 孔徑 (Å) | ~ 100 | ~ 150 | ~ 300 | ~ 500 | ~ 1,000 | ~ 2,000 |
| 蛋白分子量范圍 | 100 - 100,000 | 500 - 150,000 | 5,000 – 1,250,000 | 15,000 - 5,000,000 | 50,000 - 7,500,000 | > 10,000,000 |
| 水溶性聚合物分子量范圍 | 500-10,000 | 500-25,000 | 1,000-100,000 | 2,500-500,000 | 5,000-1,500,000 | 50,000->2,500,000 |
| pH穩(wěn)定性 | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) |
| 反壓 (psi for a 7.8x300 mm) | ~ 700 | ~ 700 | ~ 700 | ~700 | ~700 | ~700 |
| 壓力 (psi) | ~ 4,500 | ~ 4,500 | ~ 3,500 | ~ 3,000 | ~ 3,000 | ~ 3,000 |
| 鹽濃度范圍 | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M |
| 使用溫度 | ~ 80oC | ~ 80oC | ~ 80oC | ~ 80 oC | ~ 80 oC | ~ 80 oC |
| 流動相兼容性 | Aqueous and organic | Aqueous and organic | Aqueous and organic | Aqueous and organic | Aqueous and organic | Aqueous and organic |
訂購信息
Sepax SRT SEC-100
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號 |
| SRT SEC-100 | 5um | 100 Å | 21.5x600mm | 215100-21560 |
| SRT SEC-100 | 5um | 100 Å | 21.5x300mm | 215100-21530 |
| SRT SEC-100 | 5um | 100 Å | 7.5x600mm | 215100-7560 |
| SRT SEC-100 | 5um | 100 Å | 7.8x300mm | 215100-7830 |
| SRT SEC-100 | 5um | 100 Å | 7.8x150mm | 215100-7815 |
| SRT SEC-100 | 5um | 100 Å | 7.8x50 (Guard) | 215100-7805 |
| SRT SEC-100 | 5um | 100 Å | 4.6x300mm | 215100-4630 |
| SRT SEC-100 | 5um | 100 Å | 4.6x150mm | 215100-4615 |
| SRT SEC-100 | 5um | 100 Å | 4.6x50 (Guard) | 215100-4605 |
Sepax SRT SEC-150
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號 |
| SRT SEC-150 | 5um | 150 Å | 21.5x600mm | 215150-21560 |
| SRT SEC-150 | 5um | 150 Å | 21.5x300mm | 215150-21530 |
| SRT SEC-150 | 5um | 150 Å | 7.5x600mm | 215150-7560 |
| SRT SEC-150 | 5um | 150 Å | 7.8x300mm | 215150-7830 |
| SRT SEC-150 | 5um | 150 Å | 7.8x150mm | 215150-7815 |
| SRT SEC-150 | 5um | 150 Å | 7.8x50 (Guard) | 215150-7805 |
| SRT SEC-150 | 5um | 150 Å | 4.6x300mm | 215150-4630 |
| SRT SEC-150 | 5um | 150 Å | 4.6x150mm | 215150-4615 |
| SRT SEC-150 | 5um | 150 Å | 4.6x50 (Guard) | 215150-4605 |
Sepax SRT SEC-300
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號 |
| SRT SEC-300 | 5um | 300 Å | 21.5x600mm | 215300-21560 |
| SRT SEC-300 | 5um | 300 Å | 21.5x300mm | 215300-21530 |
| SRT SEC-300 | 5um | 300 Å | 7.5x600mm | 215300-7560 |
| SRT SEC-300 | 5um | 300 Å | 7.8x300mm | 215300-7830 |
| SRT SEC-300 | 5um | 300 Å | 7.8x150mm | 215300-7815 |
| SRT SEC-300 | 5um | 300 Å | 7.8x50 (Guard) | 215300-7805 |
| SRT SEC-300 | 5um | 300 Å | 4.6x300mm | 215300-4630 |
| SRT SEC-300 | 5um | 300 Å | 4.6x150mm | 215300-4615 |
| SRT SEC-300 | 5um | 300 Å | 4.6x50 (Guard) | 215300-4605 |
Sepax SRT SEC-500
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號 |
| SRT SEC-500 | 5um | 500 Å | 21.5x600mm | 215500-21560 |
| SRT SEC-500 | 5um | 500 Å | 21.5x300mm | 215500-21530 |
| SRT SEC-500 | 5um | 500 Å | 7.5x600mm | 215500-7560 |
| SRT SEC-500 | 5um | 500 Å | 7.8x300mm | 215500-7830 |
| SRT SEC-500 | 5um | 500 Å | 7.8x150mm | 215500-7815 |
| SRT SEC-500 | 5um | 500 Å | 7.8x50 (Guard) | 215500-7805 |
| SRT SEC-500 | 5um | 500 Å | 4.6x300mm | 215500-4630 |
| SRT SEC-500 | 5um | 500 Å | 4.6x150mm | 215500-4615 |
| SRT SEC-500 | 5um | 500 Å | 4.6x50 (Guard) | 215500-4605 |
Sepax SRT SEC-1000
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號 |
| SRT SEC-1000 | 5um | 1000 Å | 21.5x600mm | 215950-21560 |
| SRT SEC-1000 | 5um | 1000 Å | 21.5x300mm | 215950-21530 |
| SRT SEC-1000 | 5um | 1000 Å | 7.5x600mm | 215950-7560 |
| SRT SEC-1000 | 5um | 1000 Å | 7.8x300mm | 215950-7830 |
| SRT SEC-1000 | 5um | 1000 Å | 7.8x150mm | 215950-7815 |
| SRT SEC-1000 | 5um | 1000 Å | 7.8x50 (Guard) | 215950-7805 |
| SRT SEC-1000 | 5um | 1000 Å | 4.6x300mm | 215950-4630 |
| SRT SEC-1000 | 5um | 1000 Å | 4.6x150mm | 215950-4615 |
| SRT SEC-1000 | 5um | 1000 Å | 4.6x50 (Guard) | 215950-4605 |
Sepax SRT SEC-2000
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號 |
| SRT SEC-2000 | 5um | 2000 Å | 21.5x600mm | 215980-21560 |
| SRT SEC-2000 | 5um | 2000 Å | 21.5x300mm | 215980-21530 |
| SRT SEC-2000 | 5um | 2000 Å | 7.5x600mm | 215980-7560 |
| SRT SEC-2000 | 5um | 2000 Å | 7.8x300mm | 215980-7830 |
| SRT SEC-2000 | 5um | 2000 Å | 7.8x150mm | 215980-7815 |
| SRT SEC-2000 | 5um | 2000 Å | 7.8x50 (Guard) | 215980-7805 |
| SRT SEC-2000 | 5um | 2000 Å | 4.6x300mm | 215980-4630 |
| SRT SEC-2000 | 5um | 2000 Å | 4.6x150mm | 215980-4615 |
| SRT SEC-2000 | 5um | 2000 Å | 4.6x50 (Guard) | 215980-4605 |
注：以上為常備規(guī)格訂貨信息，其它規(guī)格或特殊定制產(chǎn)品，電詢。
