??????????????????
MiniFluo ???????????????????????????????????????,???????????????????????????
??????????е? MSP ????????????????????????????
???????????????????????????????????????,??????????????MSP??в??,????????
????;
1. 4.3??紥????,????С?????á?
2. DNA??RNA????????3??????????????
3. ?????? ??zui??DNA??????0.5pg/ul????
4. ????????????????????????????????
5. ????????Χ - ???????????
6.??????-???????????????????????????
7. ?????1000???????????USB?????????
??????;
0.5ml qPCR ????????
0.2ml qPCR ????????
MiniFluo ???????
dsDNA???????????????:0.2--100ng
ssDNA???????????????:1--200ng
???????DNA?????????:100--1600ng
????????????????
???????;
??? | MiniFluo-1 | MiniFluo-2 | MiniFluo-3 |
??? | ???? | ||
?????Χ | ????????? | ||
????????Χ | R > 0.995 | ||
????? | <1.5% | ||
??? | <1.5% | ||
????????DNA | 0.5???/???? | ||
??????? | 3s(???) | ||
???(??x??x??) | 194x155x72.5mm | ||
???? | 0.4???? |
??? | ?? | ????????? | ????????? |
MiniFluo-1 | ?????? | 365??20nm | 420-480nm(60nm) |
????? | 460??20nm | 525-570nm(45nm) | |
MiniFluo-2 | ????? | 460??20nm | 525-570nm(45nm) |
????? | 625??10nm | 670-725????(55????) | |
MiniFluo-3 | ????? | 460??20nm | 525-570nm(45nm) |
????? | 525??20nm | 575-640nm(65nm) |