人基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒英文名稱(chēng)Human Stromal cell derived factor 1a,SDF-1a ELISA試劑盒價(jià)格:電議,咨詢(xún)?cè)诰€客服,或者以郵件形式發(fā)到我司qysw@qiyibio.com。齊一生物科技(上海)有限公司提供大量品牌進(jìn)口原裝、以及穩(wěn)定性強(qiáng)的國(guó)產(chǎn)ELISA試劑盒
人基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒產(chǎn)品規(guī)格:96T/48T
產(chǎn)品庫(kù)存:現(xiàn)貨
產(chǎn)品價(jià)格:詢(xún)價(jià)
使用范圍:僅供科研檢測(cè),不得用于臨床.
保存條件:2-8℃
發(fā)貨方式:專(zhuān)門(mén)快遞免費(fèi)送貨上門(mén)
產(chǎn)品價(jià)格:*,洽談!
說(shuō)明書(shū):說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線銷(xiāo)售人員索取.全國(guó):代理多種品牌進(jìn)口原裝、分裝“ELISA試劑盒".齊一生物科技有限公司作為酶聯(lián)免疫供應(yīng)商,憑借*的技術(shù),*的售前,售中,售后免費(fèi)代測(cè)服務(wù),共同鑄就高品質(zhì)的產(chǎn)品.多年專(zhuān)業(yè)酶免服務(wù),質(zhì)量保證,可免費(fèi)提供代測(cè)服務(wù),一周內(nèi)出結(jié)果.咨詢(xún)訂購(gòu).
ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時(shí)加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個(gè)樣品根據(jù)自己的數(shù)量來(lái)定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1:1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的*標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時(shí).
甩去孔內(nèi)液體,每孔加滿(mǎn)洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿(mǎn)洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測(cè)定結(jié)果.
在450nm波長(zhǎng)處測(cè)定各孔的OD值.
31453 Pierce(R) Mouse Transferrin Peroxidase Conjugated, 1 mg 1 MG
31455 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG (Fc) 1.5 ML
31456 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgM (u) 1.5 ML
31457 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG + IgM 1.5 ML
31458 Pierce(R) Peroxidase Conj. Donkey Anti-Rabbit IgG(H+L) 0.5 ML
31460 Pierce(R) Goat Anti-Rabbit IgG, (H&L), Peroxidase Conjugated 2 ML
31461 Pierce(R) Peroxidase Conjugat ed Goat Anti-Rabbit IgG {F(ab' 2.0 ML
31462 Pierce(R) Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) 1.5 ML
31463 Pierce(R) Peroxidase Conjugat ed Goat Anti-Rabbit IgG (Fc) 2.0 ML
31464 Pierce(R) Peroxidase Conjugated Mouse Anti-Rabbit IgG (H+L) 1.0 ML
31465 ImmunoPure Human IgG, Whole Molecule-Peroxidase Conjugated, 1 MG
31466 ImmunoPure Peroxidase Conjugated Goat Anti-Rabbit 0.2 ML
31470 Pierce(R) Goat Anti-Rat IgG, (H&L), Peroxidase Conjugated, 2 ML
31471 ImmunoPure Peroxidase Conjugated Goat Anti-Rat 0.2 MG
31474 Pierce(R) Peroxidase Conjugated Goat Anti-Rat IgG F(ab')2, 2 ML
31475 Pierce(R) Peroxidase Conjugated Goat Anti-Rat IgG (Fc) 2.0 ML
31476 Pierce(R) Peroxidase Conjugated Goat Anti-Rat IgMu, 2 ML 2 ML
31480 Pierce(R) Peroxidase Conjugated Rabbit Anti-Sheep IgG (H+L), 1.5 ML
31482 ImmunoPure (R) Peroxidase Conjugated Goat Anti-Human IgG 2 ML
31485 EZ-Link Maleimide Activated Horseradish Peroxidase 5 mg
31487 EZ-Link Plus Activated Peroxidase 1 mg
31488 EZ-Link Plus Activated Peroxidase 5 X 1 mg
31489 EZ-Link Plus Activated Peroxidase Kit Kit
31490 Horseradish Peroxidase (HRP) 10 mg
31491 Horseradish Peroxidase 100 mg
31494 EZ-Link Maleimide Activated Horseradish Peroxidase Kit Kit
31497 EZ-Link Activated Peroxidase Antibody Labeling Kit Kit
31499 Recombinant Protein G, Peroxidase Conjμgated 0.5 mg
31501 Pierce(R) Fluorescein Conjugated Rabbit Anti-Chicken IgY (H 1.5 MG
31503 Pierce Peroxidase Conjμgate Stabilizer 25 mL
31505 ImmunoPure Mouse IgG, Whole Molecule- Fluorescein Conjugate 1 MG
31509 Pierce(R) Fluorescein Conjugated Rabbit Anti-Goat IgG, 1.5 MG
31512 Pierce(R) Fluorescein Conjugated Mouse Anti-Goat IgG (H&L)mi 1.0 MG
31529 Pierce(R) Fluorescein Conjugated Goat Anti-Human IgG (H+L), 2 MG
31531 Pierce(R) Fluorescein Conjugated Goat Anti-Human IgG (H+L), 1.5 MG
31533 Pierce(R) Fluorescein Conjugated Rabbit Anti-Goat IgG (Fc) 1.5 MG
31535 Pierce(R) Fluorescein Conjugated Rabbit Anti-Human IgG (Fc) 1.5 MG
31539 Pierce(R) Fluorescein Conjugated F(ab')2 Fragment Goat Anti 1 MG
31541 ImmunoPure Fluorescein Conjugated Goat Anti-Mouse IgG (H+L)( 1.5 MG
31543 Pierce(R) Fluorescein Conjuga ted Goat Anti-Mouse IgG F(ab') 2 MG
31547 Pierce(R) Fluorescein Conjuga ted Goat Anti-Mouse IgG (Fc) 2.0 MG
31553 Pierce(R) Fluorescein Conjugated Rabbit Anti-Goat IgG 1.5 MG
31555 Pierce(R) Fluorescein Conjugated Rabbit Anti-Mouse IgG (Fc) 1.5 MG
31557 Pierce(R) Fluorescein Conjugated Rabbit Anti-Mouse IgM (u) 1.5 MG
31559 Pierce(R) Fluorescein Conjugated Rabbit Anti-Mouse IgG 1.5 MG
31561 Pierce(R) Fluorescein Conjugated Rabbit Anti-Mouse IgG (H+L 1.5 MG
31565 Pierce(R) Fluorescein Conjugated F(ab')2 Fragment Goat Anti 1 MG
31568 Pierce(R) Fluorescein Conjugated Donkey Anti-Rabbit IgG (H+ 0.5 MG
31569 Pierce(R) Fluorescein Conjugated Goat Anti-Mouse IgG (H+L), 2 MG
31573 Pierce(R) Fluorescein Conjugated Goat Anti-Rabbit IgG 2.0 MG
31575 Pierce(R) Fluorescein Conjugated Goat Anti-Human IgM Fc5u, 2 MG
31577 Pierce(R) Fluorescein Conjugated Goat Anti-Human IgAa, 2 mg 2 MG
31579 Pierce(R) Fluorescein Conjugated F(ab')2 Fragment Goat Anti 1 MG
31583 Pierce(R) Fluorescein Conjugated Goat Anti-Rabbit IgG (H+L) 1.5 MG
31584 Pierce(R) Fluorescein Conjugated Mouse Anti-Rabbit IgG (H+L 1 MG
31587 ImmunoPure Rabbit Anti-Syrian Hamster IgG, (H+L) Fluorescein 1.5 MG
31620 ImmunoPure (R) Rhodamine Conjugated Mouse Anti-Goat IgG 1 MG
31621 Pierce(R) Fluorescein Conjugated Goat Anti-Rat IgG (Fc) 2.0 MG
31627 Pierce(R) Fluorescein Conjugated Rabbit Anti-Sheep IgG(H+L) 1.5 MG
31628 ImmunoPure (R) Fluorescein Conjugated Goat Anti-Human IgG 2 MG
31629 Pierce(R) Fluorescein Conjugated Goat Anti-Rat IgG (H+L), 2 2 MG
31630 ImmunoPure (R) Fluorescein Conjugated Goat Anti-Mouse IgG 1 MG
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