人T細(xì)胞急性淋巴母細(xì)胞白血病相關(guān)抗原(TALLA-1/CD231)ELISA檢測(cè)試劑盒英文名稱Human T-cell acute lymphoblastic leukemia antigen,TALLA-1 ELISA試劑盒齊一生物科技(上海)有限公司產(chǎn)品已被廣泛應(yīng)用于化學(xué)、化工、生命科學(xué)的基礎(chǔ)研究和開(kāi)發(fā)應(yīng)用、制藥、疾病診斷與控制、人口與健康、生物技術(shù)等諸多領(lǐng)域.客戶遍布國(guó)內(nèi)各大學(xué)、研究所、醫(yī)院
人T細(xì)胞急性淋巴母細(xì)胞白血病相關(guān)抗原(TALLA-1/CD231)ELISA檢測(cè)試劑盒標(biāo)本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存 過(guò)程 中 如有沉淀形成,應(yīng)再次離心。
2.血漿:應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。
3.尿液:用無(wú)菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照此實(shí)行。
4.細(xì)胞培養(yǎng)上清:檢測(cè)分泌性的成份時(shí),用無(wú)菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細(xì)收集上清。 檢測(cè)細(xì)胞內(nèi)的成份時(shí),用PBS(PH7.2-7.4)稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到100萬(wàn)/ml左右。通過(guò)反復(fù)凍融,以 使細(xì)胞破壞并 放出細(xì)胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。
5.組織標(biāo)本:切割標(biāo)本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆?。?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。分裝后一份待檢測(cè),其余冷凍備用。
標(biāo)本要求:
1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的(HRP)活性。
Human Cholic acid,CA ELISA Kit
Human Helicobacter pylori IgG ,Hp-IgG ELISA Kit
Human gamma glutamyl transpeptidase,GGT ELISA Kit
Human Gastric Mucin,GM ELISA Kit
Human Hya]uronate binding protein,HABP ELISA Kit
Human cross linked N-opeptide of type Ⅰ collagen,NTX ELISA Kit
Human Helicobacter pylori IgM,Hp-IgM ELISA Kit
Human Cytotoxin-associated protein,CagA ELISA Kit
Human gastric inhibitory polypeptide,GIP ELISA Kit
Human gastrin-reliasing peptide,GRP ELISA Kit
Human pro-gastrin-releasing peptide, ProGRP ELISA Kit
Human Collagen-like Bioprotein Ⅱ,HCBⅡ ELISA Kit
Human Secretin ELISA Kit
Human Peptide YY ELISA Kit
Human gastrin receptor,GsaR ELISA Kit
Human cholecystokinin octapeptide,CCK-8 ELISA Kit
Human trypsinogen activation peptide,TAP ELISA Kit
Human α1-Acid glycoprotein,α1-AGP ELISA Kit
Human α1-Acid glycoprotein,α1-AGP ELISA Kit
Human Motilin,MTL ELISA Kit
Human cholecystokinin,CCK ELISA Kit
Human N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit
Human Collagen Type Ⅱ,Col Ⅱ ELISA Kit
Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit
Human procollagen Ⅲ N-terminal peptide,PⅢNT ELISA Kit
Human Hyaluronic acid,HA ELISA Kit
Human Collagen Type Ⅳ,Col Ⅳ ELISA KIT
Human Laminin,LN ELISA KIT
Human anti-Nogo-A antibody,NOGO-A Ab ELISA Kit
Human anti-Survivin antibody,Surv ELISA Kit
Human anti-Granulocyte-Macrophage Colony Stimulating Factor antibody,GM-CSF Ab ELISA Kit
Human anti-presynaptic membrane antibody,PsmAb ELISA Kit
Human Angiotensin Ⅱ Receptor 2 antibody,AT2R-Ab ELISA Kit
Human angiotension Ⅱ receptor 1 Antibody,ATⅡR1 Ab ELISA Kit
Human angiotension I receptor Antibody,ANG-ⅠR antibody ELISA Kit
Human ovalbumin specific IgG,OVA sIgG ELISA Kit
Human anti-calmodulin specific antibody,CaM-ab ELISA Kit
Human thyroid hormone autoantibodies,THAA ELISA Kit
Human steroid producing cell autoantibody,SCA ELISA Kit
Human granulocyte specific antinuclear antibody,GS-ANA ELISA Kit
Human signal recognization particle antibody,SRP ELISA Kit
Human Blocking antibody,BA ELISA Kit
Human anti-cell membrane DNA antibody,cmDNA ELISA Kit
Human autoantibodies against the C-terminal domain Ⅳ,ACAST-DⅣ ELISA Kit
Human ovalbumin specific IgE,OVA sIgE ELISA Kit
Human anti-fibrillarin antibody,AFAsnoRNPU3RNP ELISA Kit
Human systemic lupus erythematosus,SLE ELISA Kit
Human anti-myelin associated glycoprotein antibody,MAG Ab ELISA Kit
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人T細(xì)胞急性淋巴母細(xì)胞白血病相關(guān)抗原(TALLA-1/CD231)ELISA檢測(cè)試劑盒豚鼠成纖維細(xì)胞生長(zhǎng)因子6 ELISA 試劑盒
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9019577 Opto switch, Z-axis, 1 ch. , Cx2
9019594 Belt, Z-axis gearbox (black), 8 ch., Cx8
9019068 PyroMark CpG SW 1.0 (5) "Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies.
Five additional software licenses for PyroMark CpG SW 1.0"
9019086 PyroMark Q96 MD Software (5) "For use with PyroMark Q96 MD instrument.Software for setting up a run and analyzing mutations.
5 licenses"
9019658 O rings - VPT Fast connector, PyroVW
972012 PyroMark Q96 CpG p16 (96) For quantification of methylation level in p16
979003 PyroMark Q96 Sample Prep Tool Cover (10) Consumables for use with magnetic tool for isolation of single stranded DNA.
979008 PyroMark Wash Buffer (conc., 200 ml) For washing and neutralizing the immobilized DNA.
950140 Binding Additive (DX) (4.0 g)
9019803 Upgrade Package 1, RCS
130803 NeXtal CubicPhase 1w Mo μplate (10)
9243044 Set, brackets, transparent panel, Qsym
283615 virotype PEDV/TGEV RT-PCR Rgt (96)
52906 QIAamp Viral RNA Mini Kit (250) For 250 RNA preps: 250 QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), RNase-free Buffers
63183 DyeEx 96 Kit (24) 24 DyeEx 96 Plates; 4 Collection Plates, 48-Well
69582 DNeasy 96 Blood & Tissue Kit (12) For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers
70042 Oligotex mRNA Midi Kit For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers
79523 Small Spin Columns 24 RNase-free spin columns for Oligotex spin procedures
301109 PolyFect Transfection Reagent (10x100ml)
9239361 PCB-micro-fuse T 6.3A, 250V, EZ1
132632 NeXtal Stock Potassium formate (50) 10M Potassium formate - 50mL (4 x 12,5 ml tube)
9019610 Cover, Pipettor, 1 ch. (white), CAS
9019624 PCB, lge breakout, 12 core to rev 3, Cx2
9019067 PyroMark CpG SW 1.0 Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies.
9019087 PyroMark IdentiFire SW 1.0 Software for sequence-based species identification by comparing and matching Pyrosequencing data to a local database.
972052 PyroMark Q96 CpG Prader Willi (96) For quantification of methylation level in Prader-Willi and Angelman Syndromes
979002 PyroMark Q96 Plate Low (100) For sample analysis of DNA template on ID.
979004 PyroMark Q96 Cartridge (3) For delivery of nucleotides and reagents for use in PyroMark Q96 ID.
9001513 PyroMark Q24 MDx For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
990610 Filter-Tips, OnCor C, 200 μl (960)
9017842 Tool, gauge, 30 mm, calibration, QIAC
9020806 Rotor Timing Calibration Dye, RGQ
331455 miScript Microfluidics PreAMP Kit (96)
381566 Investigator ESSplex SE GO! Kit (200)
9022654 Transfer Carriage QIAplate, QXT
16191 QIAwell 96 Ultra Plasmid Kit (4) For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, and QIAprep 96 Plates; Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
19046 Plasmid Buffer Set Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps
19201 Collection Tubes (2-ml) 1000 Collection Tubes (2 ml)
9015862 EZ1 DNA PRFN Card Pre-programmed card for BioRobot EZ1 paraffin section protocols
67163 MagAttract 96 DNA Plant Core Kit (24) MagAttract Suspension A and buffers for 24 x 96 preps
1030483 Elution Microtubes CL, PAX, FLU
9232687 Hood f. Tip Disposal Station 2.01
9015972 Tube Holder, STS MDx, 15 mm (12)
158926 Cell Suspension Solution (125 ml)
159992 Generation DNA Purif. Soln 1 (1000 ml)
201913 dNTP Set, PCR Grade 100mM, (4x250μl) dNTP Set is a complete set of individual, highly pure dNTPs: 100 mM each dATP, dCTP, dGTP, dTTP for 2500 x 50ul PCR reactions
12945 QIAGEN Plasmid Plus Midi Kit (100) "100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges
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9019715 PyroMark Q24 User Manual
9019861 Rotor Locking Nut Set
9019890 Foam insert, rotor securing, QIAC
336391 SureSilencing shRNA Control
330522 RT2 SYBR Green ROX qPCR Mastermix (12)