人骨骼肌受體*激酶(MUSK)檢測(cè)試劑盒(血清.血漿.組織)Huma Musclespecific tyrosine kinase, MuSK ELISA kit齊一生物科技(上海)有限公司新品*歡迎您訪問我們的如有什么問題請(qǐng)給我們或者留言741653262,告訴我您的需要,并留下您寶貴的意見和建議
人骨骼肌受體*激酶(MUSK)檢測(cè)試劑盒一、高效、靈敏、特異的抗體;
二、穩(wěn)定的重復(fù)性和可靠性;
三、吸附性能好,空白值低,孔底透明度高的固相載體;
四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型;
五、節(jié)省實(shí)驗(yàn)經(jīng)費(fèi).
elisa試劑盒回收率是反應(yīng)待測(cè)物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說你的回收率是99%,這個(gè)與真實(shí)成分有密切的關(guān)系,說明方法的準(zhǔn)確度.比如水中總無機(jī)氯含量測(cè)定,樣品水中含有無機(jī)氯20mg/L,取100mL被測(cè)水樣品,加入0.1mL濃度為10mg/mL的含無機(jī)氯標(biāo)準(zhǔn)樣品,測(cè)定時(shí)忽略體積變化,如果測(cè)定出樣品中無機(jī)氯為2.98mg/L,則認(rèn)為回收率為99%.
制備方法
包括以下步驟:
1)抗原表位的計(jì)算機(jī)篩選;
2)抗原的制備;
3)血清的采集;
4)間接ELISA方法的建立;
5)間接ELISA方法的敏感性和特異性驗(yàn)證;
6)試劑盒的穩(wěn)定性驗(yàn)證;
7)對(duì)屠宰場(chǎng)進(jìn)行臨床檢測(cè).該方法簡(jiǎn)單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出感染豬的戊型肝炎病毒,適用于大批量發(fā)病樣本的檢測(cè),可用于豬戊肝的快速診斷,并預(yù)防、控制豬戊肝病毒的流行和阻斷戊肝病毒由豬向人傳播.
組成結(jié)構(gòu)
1、血清:操作過程中避免任何細(xì)胞刺激.使用不含熱原和內(nèi)毒素的試管.收集血液后,1000×g離心10分鐘將血紅細(xì)胞迅速小心地分離.
2、血漿:EDTA、檸檬酸鹽、肝素血漿可用于檢測(cè).1000×g離心30分鐘去除顆粒.
3、細(xì)胞上清液:1000×g離心10分鐘去除顆粒和聚合物.
4、組織勻漿:將組織加入適量生理鹽水搗碎.1000×g離心10分鐘,取上清液.
5、保存:如果樣品不立即使用,應(yīng)將其分成小部分-70℃保存,避免反復(fù)冷凍.盡可能的不要使用溶血或高血脂血.如果血清中大量顆粒,檢測(cè)前先離心或過濾.不要在37℃或更高的溫度加熱解凍.應(yīng)在室溫下解凍并確保樣品均勻地充分解凍.
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1064605 MagAttract M48 DNA Manual Kit (200)
1066851 PG FMDV RT-PCR Assay (48) LE Z
1061054 HBV PCR REAGENT I
1061069 UU PCR REAGENT II
1061079 NG PCR REAGENT II
9019886 Lid, label 4CH, RG3
9019875 Cover, main, silver, RGQ
9019836 IPC Hard Drive PCA6751, PyroQ96ID
9019769 Valve, shutoff, V2, QX
9019756 Valve, solenoid, V2, QX
9019734 Sensor, Z, with cable, V2, QX
9019728 Board, motor control, Y, V2, QX
9013832 Reagent Holder, 1-bottle Holder for accommodating 1 bottle (5 ml). For use with QIAGEN Protease on the BioRobot MDx worktable
9013824 BGR Tischplatte P3041 Robotec(SN602)
9013719 RS 232 Servicecabel
9013506 96-well Thermostat Base Plate1.02
9013484 Cooling and heating system, BR3000
9013442 Centrifuge, strip adaptor set
9013434 Maintenance kit, annual, BR9600 (A)
9019723 Transport lock, EZ1xl
9019656 Hose w. quick lock socket, PyroQ96VW
9019581 Pulley, Idler Y-axis, Cxx
9019554 Rail, Z-axis 138 mm, Cxx
9019552 Rail, X-axis 444 mm, CQx
218060 miScript Reverse Transcription Kit (10)
216513 FastLane Cell Multiplex Kit (200)
204254 QuantiFast Probe PCR Kit (400)
9019440 Solid State Relay
9019433 Transformer 2x115VAC O/P 2x11VAC
9019431 Kit, lock ring 36W incl. lockknob, RG3
9019402 Transformer, heater, RG3
1027321 Mm/Hs_MAPK1 control siRNA (20 nmol)
9019329 Serial connector USB, internal cable, RG
9019238 Heating block, PyroQ24
9020478 Heating plate, GSC
1026965 PCR Buffer,10 x (1,2/2) without MgCl2
9233466 Coupling, CPC, inline, male, 6mm
9233452 Coupling, X motor, BR8000
9019217 Lead screw - Y robot, PyroQ96MD
1017635 Protease Solvent 6x (10,2/15), Azid,KG
1066846 AIV-H5 RT-PCR REAGENT LE
1054250 Effectene Transfection Reag. Kit (0.3ml)
1061051 HBV PCR REAGENT II
9019890 Foam insert, rotor securing, QIAC
9019861 Rotor Locking Nut Set
9019821 IQ/OQ document, QS
9019768 Sensor, interlock2, with cable, QX
9019765 Belt, Y, V2, QX
9019727 Board, transport conversion, V2, QX
9013523 Underlay, vaccum manifold, v2.01
9013447 Control unit Y/Z, 4 channel
9013431 Maintenance kit, semi annual, BR9600 (E)
9238807 Round carrier for 12 Tubes 15ml
9019715 PyroMark Q24 User Manual
9019648 Mixer module w. top plate, PyroQ96ID
9019643 Sensor, solenoid, EZ1xl
9019589 Cable harness, x axis, C34
9019587 PCB, mainboard, QGY
9019582 End cap, Y-arm, Cxx
9019551 Fuse, 2A
9019513 Adapter, reagent tub plate, CX1
9019502 TANK UPPER BUFFER ASSY GS
218073 miScript SYBR Green PCR Kit (200)
218061 miScript Reverse Transcription Kit (50)
217504 miRNeasy FFPE Kit (50)
9019441 Mother board ( micro board V 2.1 )