輪狀病毒抗原(RVAg)檢測試劑盒Human Rotavirus antigen,RV Ag ELISA Kit齊一生物科技有限公司新品*,齊一生物科技有限公司*經(jīng)營大鼠ELISA試劑盒,人elisa試劑盒,小鼠elisa試劑盒等種屬試劑盒,歡迎您訪問我們的,www.qiyibio。。com全國:歡迎選購!如有什么問題請給我們或者留言,告訴我您的需要
輪狀病毒抗原(RVAg)檢測試劑盒類別:酶聯(lián)免疫分析檢測試劑盒
規(guī)格:96T/48T
包裝:盒
檢測原理:采用雙抗夾心法
保質(zhì)期:六個(gè)月
產(chǎn)品保存條件:2-8°C
應(yīng)用范圍:僅供科研研究實(shí)驗(yàn)使用
樣品形式:血清/血漿/組織勻漿/細(xì)胞培養(yǎng)上清//其它生物液體(具體情況請咨詢)
標(biāo)本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存 過程 中 如有沉淀形成,應(yīng)再次離心。
2.血漿:應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照此實(shí)行。
4.細(xì)胞培養(yǎng)上清:檢測分泌性的成份時(shí),用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細(xì)收集上清。 檢測細(xì)胞內(nèi)的成份時(shí),用PBS(PH7.2-7.4)稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到100萬/ml左右。通過反復(fù)凍融,以 使細(xì)胞破壞并 放出細(xì)胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
5.組織標(biāo)本:切割標(biāo)本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆谩?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。分裝后一份待檢測,其余冷凍備用。
標(biāo)本要求:
1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
9238699 Frontpanel w. keyboard foil (Zent1)
9231793 Motor, gripper, handler, BR8000
9238690 Computer board Zent2, no Eprom
9231434 Probe, 0,4mm, ceramic coated for PK80
9020071 Keypad, TLyser
9020070 Controller 100-120V/200-240V, TLyser
9014996 Shaker, Highspeed Unit, BR8 RD
9014870 X-Motor, BR8
9014790 Tip Disposal Bags MDx Autoclavable bags for collection and disposal of used disposable tips, pack of 15
9014642 System Reagent Troughs (Qty 10)
9019188 Mirror, PyroQ96
1064181 EGFR RGQ PCR Kit (24) IUO
1017647 Carrier RNA(poly A) 12x1350μg-15-25°C,KG
1059454 ResPlex II Plus Panel (25) PRE
1055628 TopElute Fluid (60/60),KG
1061068 Sample Preparation Reagents, 30ml
1061104 UU PCR REAGENT I
9016583 ZLV Pipetting Posit., RCS1
9016561 BR MDx DSP Serv.pro Plasticware Kit, V1
9016505 Rect. Bucket Set (4)
9016486 Cable Dilutor bus, RS485, Kloehn
9016288 Cable, RS232, EZ1a
9019181 PCB 7 - Connection, PyroQ96MD
9019115 Fan - IPC, PyroQ96ID
9019101 Rotor-Gene Q Software
9019079 PyroMark Assay Design SW 2.0 (5) "All-in-one software for design of both PCR and sequencing primers
Five additional software licenses for PyroMark AssayDesign SW 1.0"
9019071 PyroMark Thermoplate, PyroQ96MD Holder for placement of PyroMark Q96 Plate on heat block during annealing step prior to PyroMark Q96 MD analysis.
9019017 Tray, EZ1xl
9019008 Reflector, UV lamp, EZ1xl
9018934 Adapter, 24x5ml flat base Tubes
9016014 X-Motor DC, Gear, 20V
9016003 BioSprint 15 tube tray "he 5-tube strips sit on this tray during the run.
see page 33 of the user manual for visuals"
9015972 Tube Holder, STS MDx, 15 mm (12)
9015910 Luminex Maintenance Kit
9015862 EZ1 DNA PRFN Card Pre-programmed card for BioRobot EZ1 paraffin section protocols
9015687 Rings (10), pinching, 4.5/2.7 mm
9015613 Cable, Interface, 3000 mm, PC to X-main
9015606 Upgrade, LiquiChip IS 2.x
9015536 Door, complete
9019165 Lead screw - Y, PyroQ96MD
9019163 Reagent chamber lid, PyroQ96MD
9013928 Channeling Adapter BR8000
9013865 QIAsoft 4.x, BR8000 Cust. Edition
9021191 Adapter, deep-well plate, BS96
9021052 Controller, Galil Motion, DCU
9021009 Cap, Reagent Bottle, DCU
9021005 Motor, Pipettor Z, DCU
9020911 Foot assembly, SMC, QGX
9020716 Optoswitch, X axis, C34
1061117 HSV PCR REAGENT I
9016566 Syringe Pump, RCS1
9016487 Cable REDGE/Gateway, RS232, Kloehn
9016306 Computer, standard, XP
9016233 Hardware Upgrade Kit, Universal Tissue
9019183 Valve - Release, PyroQ96
Instrument Part 9241163 Board, motor control, X, QX
Instrument Part 9018473 Transport box, QIAC
Instrument Part 9017656 Adapter Set, press.sensor, BR8 RD
Instrument Part 9020169 Rotor Hub Assy, RG3
Instrument Part 9020197 PCB, breakout, P-axis, 8 ch. , QX1
Instrument Part 9020160 Insulation disc - chamber side, RG3
Consumable 218160 miScript II RT Kit (12)
Consumable 330504 RT2 SYBR Green qPCR Mastermix (6)
Instrument Part 9238673 Hinge, imbalance system
Instrument Part 9238690 Computer board Zent2, no Eprom
Instrument Part 9238699 Frontpanel w. keyboard foil (Zent1)
Instrument Part 9238716 Seal for lid, 1350 mm
Instrument Part 9238794 Angle rotor 6 x 250 ml
Instrument Part 9238798 Rectangular bucket, sealable with cap
Instrument Part 9238817 Rect. carrier for 12 glass tubes 15ml
Instrument Part 9238827 Rotor w/o bucket
Instrument Part 9014004 Light, Barrier, Drawer, BR8 RD
Instrument Part 9014642 System Reagent Troughs (Qty 10)
Instrument Part 9016913 Tubing, press relief valve inlet DISU v2
Instrument Part 9239812 Holder strap, VacTrap, BR8
Consumable 129056 MaXtract High Density (200 x 2 ml)
Consumable 129065 MaXtract High Density (100 x 15 ml)
Instrument Part 9017785 Board, Y, QIAC
Consumable 337841 qBiomarker SYBR ROX FAST Mastermix (8)
Consumable 59864 EpiTect Fast LyseAll Bisulfite Kit (50)
Instrument Part 9013723 Motor-X, RCS1
Instrument Part 9016001 Magnet head for medium 96 plate, BS96
Instrument Part 9016270 Maintenance Kit, Multiflow BR MDx V2
Instrument Part 9016265 MJR Microseal Pad for ALP 2296 MSP-1002
Instrument Part 9238612 Collet chuck screw
Instrument Part 9238635 Hook identification compl.
Instrument Part 9238653 Power Board, temp. switch - heat sink
Consumable 204541 QuantiTect Multiplex PCR Kit (40)
Instrument Part 9021025 Waste bag holder, AS, Qsym
Instrument Part 9021097 Sensor, Proximity SME-8M, DCU
Consumable 180984 GeneRead Adapter I Set 12plex (72)
Consumable 9243772 QIAsymphony AS Custom Protocol Basic1
Consumable 971510 therascreen? GIST RapidScreen Pyro Kit
Instrument Part 9238691 Relay 2U 24 V
Instrument Part 9238702 Imbalance switch, magnetic
Instrument Part 9238769 Swing-out rotor 48 x 15 ml complete
Instrument Part 9238786 Angle rotor 100 x 1.5 / 2.2 ml, incl. al
Instrument Part 9238816 Rect. carrier for 12 Vacutainertubes 7ml
Instrument Part 9238823 Rect. carrier for 1 bottle 200 ml w/ cap
Instrument Part 9014638 System Syringes (Qty 4)
Instrument Part 9235699 Power Cable, RCS1
Instrument Part 9236941 SlideShett, SamTrak MDx
9240097 BR, Re-Installation
Instrument Part 9016839 Locking mechanism, RoboVac base v4.2
Instrument Part 9017354 Holder, VacTrap, BR8
Instrument Part 9239106 Grub screw set, Kloehn syringe lock (10)
Instrument Part 9239568 Needle, W-Drive, DISU, BR8, v2.0
Consumable 129046 MaXtract High Density (200 x 1.5 ml)