表皮生長因子試劑盒_EGF酶聯(lián)免疫分析
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性,在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。
表皮生長因子試劑盒_EGF酶聯(lián)免疫分析操作步驟(僅供參考具體詳見說明書)
實(shí)驗(yàn)開始前,請(qǐng)?zhí)崆芭渲煤盟性噭?,試劑或樣品稀釋時(shí),均需混勻,混勻時(shí)盡量避免起泡。每次檢測(cè)都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過高時(shí),用樣品稀釋液進(jìn)行稀釋,以使樣品符合試劑盒的檢測(cè)范圍。
1.加樣:分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測(cè)樣品孔??瞻卓准訕悠废♂屢?00μl,余孔分別加標(biāo)準(zhǔn)品或待測(cè)樣品100μl,注意不要有氣泡,加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻,酶標(biāo)板加上蓋或覆膜,37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性,每次實(shí)驗(yàn)請(qǐng)使用新的標(biāo)準(zhǔn)品溶液。
2. 棄去液體,甩干,不用洗滌。每孔加*標(biāo)記抗體工作液 100μl(取1μl*標(biāo)記抗體加99μl*標(biāo)記抗體稀釋液的比例配制,輕輕混勻,在使用前一小時(shí)內(nèi)配制),37℃,60分鐘。
3.溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板3次,每次浸泡1-2分鐘,350μl/每孔,甩干。
4.每孔加辣根過氧化物酶標(biāo)記親和素工作液(同*標(biāo)記抗體工作液) 100μl,37℃,60分鐘。
5.溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板5次,每次浸泡1-2分鐘,350μl/每孔,甩干。
6.依序每孔加底物溶液90μl,37℃避光顯色(30分鐘內(nèi),此時(shí)肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色,后3-4孔梯度不明顯,即可終止)。
7.依序每孔加終止溶液50μl,終止反應(yīng)(此時(shí)藍(lán)色立轉(zhuǎn)黃色)。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性,底物反應(yīng)時(shí)間到后應(yīng)盡快加入終止液。
8.用酶聯(lián)儀在450nm波長依序測(cè)量各孔的光密度(OD值)。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測(cè)。
注:
1. 用戶在初次使用試劑盒時(shí),應(yīng)將各種試劑管離心數(shù)分鐘,以便試劑集中到管底。
2. 每次實(shí)驗(yàn)留一孔作為空白調(diào)零孔,該孔不加任何試劑,只是zui后加底物溶液及2N H2SO4。測(cè)量時(shí)先用此孔調(diào)OD值至零。
3. 為防止樣品蒸發(fā),試驗(yàn)時(shí)將反應(yīng)板放于鋪有濕布的密閉盒內(nèi),酶標(biāo)板加上蓋或覆膜。
4. 未使用完的酶標(biāo)板或者試劑,請(qǐng)于2-8℃保存。標(biāo)準(zhǔn)品、*標(biāo)記抗體工作液、辣根過氧化物酶標(biāo)記親和素工作液請(qǐng)依據(jù)所需的量配置使用。請(qǐng)勿重復(fù)使用已稀釋過的標(biāo)準(zhǔn)品、*標(biāo)記抗體工作液或、辣根過氧化物酶標(biāo)記親和素工作液。
5. 建議檢測(cè)樣品時(shí)均設(shè)雙孔測(cè)定,以保證檢測(cè)結(jié)果的準(zhǔn)確性。
表皮生長因子試劑盒_EGF酶聯(lián)免疫分析試劑盒安全性提示:
1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3.不要用嘴吸取試劑盒里的任何成份
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CaMK2b(calcium/calmodulin-dependent protein kinase II beita) 鈣/鈣調(diào)素依賴蛋白激酶2b抗體
CAP1/Park7/DJ-1 Park7/DJ-1/CAP1抗體
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QY229-1 碳酸銀 Silver carbonate 534-16-7 CP,98% "儲(chǔ)存條件: RT,避光
介紹:
新沉淀時(shí)為亮黃色,干燥后變?yōu)閹Щ尹S色,在光線下色澤暗,久置色也變暗,感光性*。約在220℃時(shí)分解為氧化銀和二氧化碳,更高溫度時(shí)分解為金屬銀。水,易溶于稀硝酸、乙酸、*溶液、氟化鉀、氨水和氰化堿溶液,不溶于冷水和醇。有刺激性。新制得的為淺黃色粉未。溶于氨水、稀硝酸、,不溶于水和醇。避光保存。"
QY230-1 碘化銀 Silver iodide 7783-96-2 CP "熔點(diǎn): 557°C
沸點(diǎn): 1506°C
比重: 5·68
儲(chǔ)存條件: RT,避光
溶解性: 易溶于氰化堿和碘化堿溶液,溶于濃的氫碘酸、溴化堿、氯化堿、硫氰酸堿、硫代硫酸堿、*溶液,幾乎不溶于水、氫碘酸以外的酸和碳酸銨溶液
用途: 微量分析銫。人工降雨。攝影。制藥。
敏感性: 對(duì)光敏感
介紹: 無氣味。遇空氣與光逐漸變?yōu)楹谏D芫徛嘏c沸的濃酸反應(yīng),而不與熱的氫氧化堿溶液反應(yīng)。"
QY231-1 硫酸銀 Silver sulfate 10294-26-5 AR,99.7% "熔點(diǎn): 652°C
沸點(diǎn): 1085°C
比重: 5·45
儲(chǔ)存條件: RT,避光
溶解性: 能溶于硝酸、氨水及濃硫酸和熱水,,不溶于乙醇。能慢慢地溶于150分水和71分沸水。
用途: 比色測(cè)定硝酸鹽、磷酸鹽和氟,測(cè)定乙烯,水質(zhì)分析中鉻和鈷的測(cè)定。
敏感性: 對(duì)光線敏感
介紹:
在光線下逐漸變黑色。在1085度分解。"
QY232-1 乙酸銀 Silver acetate 563-63-3 CP,99% "儲(chǔ)存條件: RT,避光
溶解性: 易溶于稀硝酸,溶于100份冷水、35份沸水。
敏感性: 對(duì)光敏感
介紹: 對(duì)光敏感。有刺激性"
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儲(chǔ)存條件: RT
敏感性: 對(duì)光敏感
介紹: 不溶于水,溶于HCl、HNO3、NaCN溶液中"
QY234-1 2,5-二苯惡唑 PPO 92-71-7 BR,99% "熔點(diǎn): 72°C
沸點(diǎn): 360°C
比重: 1·06
儲(chǔ)存條件: RT,避光
溶解性: 溶于醇、醚和石油醚,不溶于水。
用途: 該品是閃爍試劑,用于閃爍光譜。
介紹: 能隨水蒸氣微揮發(fā)。"
QY235-1 1,4-雙(5-苯基-2-惡唑)苯 POPOP 1806-34-4 AR "熔點(diǎn): 242-246
儲(chǔ)存條件: RT
溶解性: 不溶于水、乙醇,微溶于甲苯,"
QY236-1 咪唑 Imidazole 288-32-4 AR,99%,腐蝕品 "熔點(diǎn): 90-91°C
沸點(diǎn): 257°C
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儲(chǔ)存條件: RT"
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PIK3CD Antibody O00329 50ug/50ul ,100ug/100ul Human WB IF/ICC
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NEUROG2 Antibody Q9H2A3 50ug/50ul ,100ug/100ul Human WB
PKMYT1 Antibody Q99640 50ug/50ul ,100ug/100ul Human WB
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YY1 Antibody P25490 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
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MSLN Antibody Q13421 50ug/50ul ,100ug/100ul Human WB
LCAT Antibody P04180 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
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A2M Antibody P01023 50ug/50ul ,100ug/100ul Human WB
UFM1 Antibody P61960 50ug/50ul ,100ug/100ul Human, Mouse WB
TRPM8 Antibody Q7Z2W7 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
IRF9 Antibody Q00978 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
ATG12 Antibody O94817 50ug/50ul ,100ug/100ul Human WB
CARD11 Antibody Q9BXL7 50ug/50ul ,100ug/100ul Human WB
ATG12 Antibody O94817 50ug/50ul ,100ug/100ul Human WB
ZBTB33 Antibody Q86T24 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
EHD1 Antibody Q9H4M9 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
FDX1 Antibody P10109 50ug/50ul ,100ug/100ul Human WB
SIX2 Antibody Q9NPC8 50ug/50ul ,100ug/100ul Human WB
ICAM3 Antibody P32942 50ug/50ul ,100ug/100ul Human WB
BNIP3 Antibody Q12983 50ug/50ul ,100ug/100ul Human WB
BGN Antibody P21810 50ug/50ul ,100ug/100ul Human WB IHC
XPO4 Antibody Q9C0E2 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC IF/ICC
NUDC Antibody Q9Y266 50ug/50ul ,100ug/100ul Human WB IF/ICC
RBBP7 Antibody Q16576 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
UBE2N Antibody P61088 50ug/50ul ,100ug/100ul Human WB
ENTPD2 Antibody Q9Y5L3 50ug/50ul ,100ug/100ul Human WB
NETO2 Antibody Q8NC67 50ug/50ul ,100ug/100ul Human, Mouse WB
SOX1 Antibody O00570 50ug/50ul ,100ug/100ul Human WB IHC
FADD Antibody Q13158 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
SENP3 Antibody Q9H4L4 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
CHRNA4 Antibody P43681 50ug/50ul ,100ug/100ul Human WB
OSR1 Antibody Q8TAX0 50ug/50ul ,100ug/100ul Human WB IHC
PTRH2 Antibody Q9Y3E5 50ug/50ul ,100ug/100ul Human, Mouse WB
STXBP1 Antibody P61764 50ug/50ul ,100ug/100ul Human WB IHC
UCHL5 Antibody Q9Y5K5 50ug/50ul ,100ug/100ul Human WB
CDK5R2 Antibody Q13319 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
UGGT1 Antibody Q9NYU2 50ug/50ul ,100ug/100ul Human WB
TAF9B Antibody Q9HBM6 50ug/50ul ,100ug/100ul Human, Mouse WB
SCRIB Antibody Q14160 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
PLA2G1B Antibody P04054 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
SLC4A1 Antibody P02730 50ug/50ul ,100ug/100ul Human WB
CDC6 Antibody Q99741 50ug/50ul ,100ug/100ul Human WB
FLII Antibody Q13045 50ug/50ul ,100ug/100ul Human WB
DAZL Antibody Q92904 50ug/50ul ,100ug/100ul Human WB
MLLT11 Antibody Q13015 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
CD48 Antibody P09326 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
URM1 Antibody Q9BTM9 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
ATG3 Antibody Q9NT62 50ug/50ul ,100ug/100ul Human WB
NODAL Antibody Q96S42 50ug/50ul ,100ug/100ul Human, Mouse WB
FDX1 Antibody P10109 50ug/50ul ,100ug/100ul Human, Mouse WB
ITCH Antibody Q96J02 50ug/50ul ,100ug/100ul Human WB
NUDC Antibody Q9Y266 50ug/50ul ,100ug/100ul Human, Mouse WB
UBE2N Antibody P61088 50ug/50ul ,100ug/100ul Human, Mouse WB
USP28 Antibody Q96RU2 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
HDLBP Antibody Q00341 50ug/50ul ,100ug/100ul Human WB IHC
ITCH Antibody Q96J02 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
S100A2 Antibody P29034 50ug/50ul ,100ug/100ul Human WB IHC
SSTR5 Antibody P35346 50ug/50ul ,100ug/100ul Human WB
TSPO Antibody P30536 50ug/50ul ,100ug/100ul Human WB
PAM Antibody P19021 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
ACTC1 Antibody P68032 50ug/50ul ,100ug/100ul Human, Mouse WB
STXBP1 Antibody P61764 50ug/50ul ,100ug/100ul Human WB
SLC4A1 Antibody P02730 50ug/50ul ,100ug/100ul Human WB
GFPT1 Antibody Q06210 50ug/50ul ,100ug/100ul Human,Rat WB
ABCA1 Antibody O95477 50ug/50ul ,100ug/100ul Human WB
RIPK1 Antibody Q13546 50ug/50ul ,100ug/100ul Human, Mouse WB
CD163 Antibody Q86VB7 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
MSLN Antibody Q13421 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
GBA Antibody P04062 50ug/50ul ,100ug/100ul Human, Mouse WB
DUSP3 Antibody P51452 50ug/50ul ,100ug/100ul Human, Mouse WB IHC
COPS8 Antibody Q99627 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
ING2 Antibody Q9H160 50ug/50ul ,100ug/100ul Human WB
TRAM2 Antibody Q15035 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
ATG3 Antibody Q9NT62 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
UBE2M Antibody P61081 50ug/50ul ,100ug/100ul Human,Rat WB IHC
SSR1 Antibody P43307 50ug/50ul ,100ug/100ul Human WB
TLN2 Antibody Q9Y4G6 50ug/50ul ,100ug/100ul Human WB
CTSS Antibody P25774 50ug/50ul ,100ug/100ul Human WB
HTT Antibody P42858 50ug/50ul ,100ug/100ul Human WB
EEA1 Antibody Q15075 50ug/50ul ,100ug/100ul Human WB
OS9 Antibody Q13438 50ug/50ul ,100ug/100ul Human WB IHC
BCAM Antibody P50895 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
GRK1 Antibody Q15835 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
TIMELESS Antibody Q9UNS1 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
CD97 Antibody P48960 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
UNC13D Antibody Q70J99 50ug/50ul ,100ug/100ul Human, Mouse WB
NR2C2 Antibody P49116 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
CDH10 Antibody Q9Y6N8 50ug/50ul ,100ug/100ul Human WB
PPP1R9A Antibody Q9ULJ8 50ug/50ul ,100ug/100ul Human WB
SEPP1 Antibody P49908 50ug/50ul ,100ug/100ul Human WB IHC
NEK6 Antibody Q9HC98 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
PNP Antibody P00491 50ug/50ul ,100ug/100ul Human WB IHC
MAPKAPK2 Antibody P49137 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
LGALS8 Antibody O00214 50ug/50ul ,100ug/100ul Human, Mouse WB
CD53 Antibody P19397 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
UIMC1 Antibody Q96RL1 50ug/50ul ,100ug/100ul Human WB
F5 Antibody P12259 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
KIF3A Antibody Q9Y496 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB IHC
CD82 Antibody P27701 50ug/50ul ,100ug/100ul Human WB
PSMA5 Antibody P28066 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
JAG1 Antibody P78504 50ug/50ul ,100ug/100ul Human WB
IFITM3 Antibody Q01628 50ug/50ul ,100ug/100ul Human, Mouse,Rat WB
亞麻酸含量測(cè)定試劑盒 高效液相色譜法 100T 咨詢
*含量測(cè)定試劑盒 高效液相色譜法 100T 咨詢
*含量測(cè)定試劑盒 高效液相色譜法 100T 咨詢
*含量測(cè)定試劑盒 高效液相色譜法 100T 咨詢
*3含量測(cè)定試劑盒 高效液相色譜法 100T 咨詢
*1含量測(cè)定試劑盒 高效液相色譜法 100T 咨詢
*含量測(cè)定試劑盒 高效液相色譜法 100T 咨詢
*1含量測(cè)定試劑盒 高效液相色譜法 100T 咨詢
*2含量測(cè)定試劑盒 高效液相色譜法 100T 咨詢
鹽酸水蘇堿 Stachydrine hydrochloride 4136-37-2 20mg
延齡草苷 Diosgenin glucoside 14144-06-0 20mg
原阿片堿 Protopine 130-86-9 20mg
* Etoposide 33419-42-0 20mg
鹽酸益母草堿 Leonurine hydrochloride 24697-74-3 20mg
乙酰紫堇靈 Acetylcorynoline 18797-80-3 20mg
紫堇靈 (+)-corynoline 18797-79-0 20mg
脫氫紫堇堿 Dehydrocorydaline 30045-16-0 20mg
異鼠李素--3-O-β-D-葡萄糖苷 Isorhamnetin-3-O-β-D-Glucoside 5041-82-7 20mg
鴨腳樹葉堿 2α,5α-Epoxy-1,2-dihydroakuammilan-17-oic acid methyl ester 4684-32-6 20mg
銀鍛苷 Tiliroside 20316-62-5 20mg
3-乙?;?β乳香酸 3-Acetyl-β-boswellic acid 5968-70-7 10mg
3-乙?;?1-酮基乳香酸 beta-boswellic acid,3-acetyl,11-keto 20mg
QYS-23066 尿酸含量試劑盒 微量法
QYS-23067 尿酸含量試劑盒 可見分光光度法
QYS-23068 硫氧還蛋白過氧化物酶(TPX)試劑盒 微量法
QYS-23069 硫氧還蛋白過氧化物酶(TPX)試劑盒 紫外分光光度法
QYS-23070 *過氧化物酶(GPX)試劑盒 微量法
QYS-23071 *過氧化物酶(GPX)試劑盒 GR法
QYS-23072 *S-轉(zhuǎn)移酶(GST)試劑盒 微量法
QYS-23073 *S-轉(zhuǎn)移酶(GST)試劑盒 紫外分光光度法
QYS-23074 抗壞血酸(AsA)/*含量試劑盒 微量法
QYS-23075 抗壞血酸(AsA)/*含量試劑盒 紫外分光光度法
QYS-23076 抗壞血酸(AsA)/*含量試劑盒 高效液相色譜法
QYS-23077 抗壞血酸(AsA)/*含量試劑盒 滴定法
QYS-23078 總巰基試劑盒 微量法
QYS-23079 總巰基試劑盒 可見分光光度法
QYS-23080 植物花色苷試劑盒 微量法
QYS-23081 植物花色苷試劑盒 可見分光光度法
QYS-23082 單寧含量試劑盒 微量法
QYS-23083 單寧含量試劑盒 可見分光光度法
QYS-23084 超氧陰離子清除能力試劑盒 微量法
QYS-23085 超氧陰離子清除能力試劑盒 可見分光光度法
QYS-23086 非蛋白質(zhì)巰基試劑盒 微量法
表皮生長因子(EGF)試劑盒 96次/48次 血清、血漿、組織等