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上海研盟生物科技有限公司Anti-Alpha-Synuclein抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-Alpha-Synuclein antibody
中文名稱:核突觸蛋白α抗體
核突觸蛋白α抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
★研盟生物★核突觸蛋白α抗體,Anti-Alpha-Synuclein抗體
石蠟切片的免疫組化SP法參考步驟:
1.60℃烤片2小時(shí)
2.脫蠟。二甲苯脫蠟15分鐘,三次
3.水化。依次經(jīng)過(guò)無(wú)水乙醇、95%、90%、80%、70%,各5分鐘,蒸餾水(或自來(lái)水)5分鐘
4.PBS洗5分鐘,三次
5.抗原修復(fù)。枸櫞酸緩沖液(約92-95℃)煮沸(沸水?。┬迯?fù)15分鐘,自然涼至室溫。PBS洗5分鐘,三次
6.3%雙氧水消化內(nèi)源性的過(guò)氧化物酶,20分鐘,PBS洗5分鐘,三次
7.正常山羊血清封閉,37℃20分鐘
8.一抗孵育,4℃過(guò)ye.
9.PBS洗5分鐘,三次
10.二抗孵育,37℃20分鐘(二抗為*標(biāo)記的羊抗兔IgG),PBS洗5分鐘,三次
11.三抗孵育,37℃20分鐘(三抗為辣根酶標(biāo)記的鏈霉親和素),PBS洗5分鐘,三次
12.DAB顯色,鏡下觀察結(jié)果,適時(shí)終止
13.蘇木素復(fù)染5分鐘,自來(lái)水沖洗片刻,1%的鹽酸酒精(75%)溶液(請(qǐng)自行配置,配制比例為:100ml 75%酒精+1ml濃鹽酸)分化30秒,自來(lái)水沖洗5分鐘藍(lán)化
14.脫水。依次經(jīng)過(guò)70%、80%、90%、95%、無(wú)水乙醇,各5分鐘,二甲苯15分鐘,三次
15.封片,中性樹(shù)膠封片
核突觸蛋白α抗體,Anti-Alpha-Synuclein抗體溶解方法:
方法1:我們的抗體(凍干粉)已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑(疊氮鈉或*),您只需要加入滅菌的雙蒸水就行了??贵w溶解后,置于-20℃保存,分裝后使用,避免反復(fù)凍融,可保證至少1年有效;
方法2:也可以只加入一半體積的雙蒸水,再用一半體積的甘油補(bǔ)足,置于-20℃保存,這樣抗體不會(huì)凍,有利于抗體的穩(wěn)定。
后續(xù)試驗(yàn)時(shí),再使用對(duì)應(yīng)的緩沖液稀釋成工作液,即用即配。
我們的抗體(凍干粉),在常溫放置下,至少一個(gè)月有效;若在-20℃下保存(*),至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
核突觸蛋白α抗體,Anti-Alpha-Synuclein抗體簡(jiǎn)單介紹:
產(chǎn)品別名:Alpha synuclein; Alpha-Synuclein; Alpha-synuclein, isoform NACP140; alpha SYN; MGC105443; MGC110988; MGC127560; MGC64356; NACP; Non A beta component of AD amyloid; Non A4 component of amyloid precursor; Non-A-beta component of alzheimers disease amyloid , precursor of; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK 1; PARK 4; PARK1; PARK4; Parkinson disease (autosomal dominant, Lewy body) 4; Parkinson disease familial 1; PD 1; PD1; Syn; SNCA; Snca synuclein, alpha (non A4 component of amyloid precursor); SYUA_HUMAN; Synuclein alpha; Synuclein, alpha (non A4 component of amyloid precursor); α Synuclein; α-Synuclein.
抗體來(lái)源:Rabbit or Mouse
保質(zhì)期:1年
克隆類(lèi)型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.
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