上海研盟生物科技有限公司Anti-D3R抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?
凋亡誘導(dǎo)受體TNFRSF25抗體,Anti-D3R抗體簡(jiǎn)單介紹
中文名稱:凋亡誘導(dǎo)受體TNFRSF25抗體
英文名稱:Anti-D3R antibody
英文別名:Apo 3; Apo-3; Apo3; Apoptosis inducing receptor AIR; Apoptosis inducing receptor; Apoptosis mediating receptor; Apoptosis mediating receptor DR 3; Apoptosis mediating receptor DR3; Apoptosis mediating receptor TRAMP; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; DDR 3; DDR3; Death domain receptor 3; Death Receptor 3; Death receptor beta; DR 3; LARD; Lymphocyte associated receptor of death; Lymphocyte-associated receptor of death; Protein WSL; Protein WSL-1; TNFRSF 12; TNFRSF 25; TNFRSF12; TNFRSF25; TNR25_HUMAN; TR 3; TR3; TRAMP; Translocating chain association membrane protein; Tumor necrosis factor receptor superfamily member 12; Tumor necrosis factor receptor superfamily member 25; WSL 1; WSL; WSL LR; WSL protein; WSL1; WSL1 protein; WSLLR.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實(shí)驗(yàn)
產(chǎn)品價(jià)格:請(qǐng)報(bào)價(jià)
說明書:請(qǐng)?zhí)砑涌头苯铀魅?/span>
抗體來源:該指標(biāo)有兩種產(chǎn)品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應(yīng):請(qǐng)索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲(chǔ) 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
背景介紹: The protein encoded by this gene is a member of theTNF-receptor superfamily. This receptor is expressed preferentiallyin the tissues enriched in lymphocytes, and it may play a role inregulating lymphocyte homeostasis. This receptor has been shown tostimulate NF-kappa B activity and regulate cell apoptosis. Thesignal transduction of this receptor is mediated by various deathdomain containing adaptor proteins. Knockout studies in micesuggested the role of this gene in the removal of self-reactive Tcells in the thymus. Multiple alternatively spliced transcriptvariants of this gene encoding distinct isoforms have beenreported, most of which are potentially secreted molecules. Thealternative splicing of this gene in B and T cells encounters aprogrammed change upon T-cell activation, which predominantlyproduces full-length, membrane bound isoforms, and is thought to beinvolved in controlling lymphocyte proliferation induced by T-cellactivation.
凋亡誘導(dǎo)受體TNFRSF25抗體保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
免疫組化定義:用標(biāo)記的特異性抗體對(duì)組織切片或細(xì)胞標(biāo)本中某些化學(xué)成分的分布和含量進(jìn)行組織和細(xì)胞原位定性、定位或定量研究,這種技術(shù)稱為免疫組織化學(xué)(immunohistochemistry)技術(shù)或免疫細(xì)胞化學(xué)(immunocytochemistry)技術(shù)。
免疫組化原理
根據(jù)抗原抗體反應(yīng)和化學(xué)顯色原理,組織切片或細(xì)胞標(biāo)本中的抗原先和一抗結(jié)合,再利用一抗與標(biāo)記*、熒光素等的二抗進(jìn)行反應(yīng),前者再用標(biāo)記辣根過氧化物酶(HRP)或堿性磷酸酶(AKP)等的抗*(如鏈霉親和素等)結(jié)合,zui后通過呈色反應(yīng)或熒光來顯示細(xì)胞或組織中化學(xué)成分,在光學(xué)顯微鏡或熒光顯微鏡下可清晰看見細(xì)胞內(nèi)發(fā)生的抗原抗體反應(yīng)產(chǎn)物,從而能夠在細(xì)胞爬片或組織切片上原位確定某些化學(xué)成分的分布和含量。
凋亡誘導(dǎo)受體TNFRSF25抗體,Anti-D3R抗體部分實(shí)驗(yàn)介紹:
有關(guān)免疫組化實(shí)驗(yàn)的重要問題
(一)組織材料處理
1.取材要取新鮮組織,大小合適,用生li鹽水洗凈
2.固定固定就是把病理標(biāo)本組織浸泡在固定液中(常用的有4%多聚甲醛),使組織或細(xì)胞內(nèi)蛋白質(zhì)凝聚、沉淀或變性成為不溶性物質(zhì),并保持細(xì)胞原有的形態(tài)結(jié)構(gòu)
3.洗滌固定后一定要把滲透到組織里面的固定液洗去,以利于進(jìn)行脫水,若組織中留有較多的固定液,則將影響脫水劑,甚至可在組織中發(fā)生沉淀而影響后續(xù)染色及觀察,對(duì)于陳舊性標(biāo)本或固定時(shí)間過長(zhǎng)的標(biāo)本,更應(yīng)注意流水沖洗。
4.脫水脫水必須*,若脫水不盡,則切片后組織與空氣接觸即干燥收縮,蠟塊切面出現(xiàn)凹陷現(xiàn)象,雖可勉強(qiáng)切片,但染色效果差,染色時(shí)也容易脫落
5.透明乙醇等脫水劑不能溶解石蠟,所以浸蠟之前需要一個(gè)既能與乙醇混合又能溶解石蠟的媒劑,以便使石蠟浸入到組織中去,當(dāng)組織中全部被透明劑占有時(shí),光線可以透過,組織可呈現(xiàn)不同程度的透明狀態(tài),此現(xiàn)象稱為透明。如果組織不能透明,表明脫水未盡必須重新返工,否則影響到浸蠟的進(jìn)行,但返工時(shí)往往效果不好
6.浸蠟浸蠟時(shí)間根據(jù)組織的類型可適當(dāng)調(diào)整,一般原則較小組織脫水時(shí)間和浸蠟時(shí)間較短;骨、肌肉等堅(jiān)硬組織脫水和浸蠟時(shí)間不宜過長(zhǎng);脂肪組織、疏松結(jié)締組織脫水和浸蠟時(shí)間需增加
7.包埋包埋時(shí)應(yīng)注意組織的包埋方向,否則將影響組織切片的形態(tài)。包埋用的石蠟要和浸蠟用的石蠟熔點(diǎn)*,包埋用的石蠟溫度要稍高于浸蠟的溫度。包埋好后可置于冷水中使石蠟迅速冷凝。包埋好的石蠟塊應(yīng)呈均勻的半透明狀,如出現(xiàn)白色渾濁則其中存在石蠟的結(jié)晶,這種蠟塊不能切除薄的切片??赡苁墙M織脫水不充分,組織內(nèi)或石蠟中混有透明劑,或包埋時(shí)動(dòng)作太慢或石蠟?zāi)烫?,所以冷卻時(shí)水溫不能過高,否則就不宜迅速凝固
8.切片可以將制作好的蠟塊置于低溫環(huán)境預(yù)冷,切出的切片連續(xù)性較好,有利于展片,一般切片厚度4-6um
(二)抗原修復(fù)
組織在制作過程中,由于固定液的作用封閉了抗原,又由于熱的作用致使部分抗原的肽鏈發(fā)生扭曲,致使在免疫組化的染色過程中不能將其顯示出來,為了解決上述的問題,利用化學(xué)試劑和熱的作用將這些抗原重新暴露出來或修正過來的過程稱為抗原修復(fù)。
上海研盟生物科技有限公司常用的抗原修復(fù)液有:0.01M枸櫞酸(pH6.0)、0.05M EDTA(pH8.0)、0.01M TBS(pH7.4)、*、*
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