上海研盟生物科技有限公司Anti-beta Amyloid 1-42抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細胞術(shù)等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-beta Amyloid 1-42 antibody
中文名稱:β淀粉樣肽(1-42)抗體
β淀粉樣肽(1-42)抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
β淀粉樣肽(1-42)抗體,Anti-beta Amyloid 1-42抗體抗原修復(fù)方法:
方法1:沸水浴修復(fù),將盛有修復(fù)液和玻片的燒杯置于沸水浴環(huán)境,保持外部沸騰狀態(tài)15min,自然冷卻至室溫。
方法2:微波修復(fù),將盛有修復(fù)液和玻片的燒杯置于微波爐中,高火5min,?;?min,中火5min,自然冷卻至室溫。
方法3:高壓修復(fù),修復(fù)液加入高壓鍋加熱至沸騰,放入玻片,封蓋加壓持續(xù)加熱至噴氣時開始計時修復(fù)2min,自然冷卻至室溫。
方法4:使用*修復(fù),將*修復(fù)液加入到組織上,37℃,消化30min
★研盟生物★β淀粉樣肽(1-42)抗體,Anti-beta Amyloid 1-42抗體
抗原修復(fù),必須注意以下問題:
1、修復(fù)液的選擇。
0.01M枸櫞酸(pH6.0):應(yīng)用zui多的抗原修復(fù)液,適用于大多數(shù)的抗原,用該液修復(fù)后的抗原表達增強,抗原的定性和定位很理想,結(jié)果不錯;
0.05M EDTA(pH8.0):應(yīng)用較多的抗原修復(fù)液,修復(fù)能力較強,對于保存時間較長的切片或目的蛋白表達較弱的組織有很好的修復(fù)效果,需要控制好修復(fù)時間,否則容易出現(xiàn)較重的背景;
0.01M TBS(pH7.4):中性修復(fù)液,適用于大多數(shù)抗原,對于核定位蛋白有較好的修復(fù)效果;
*:應(yīng)用較多的抗原修復(fù)液,適用于大多數(shù)的抗原,使用溫度37℃,對于容易脫片的切片有很好的保護作用。
*:應(yīng)用較多的抗原修復(fù)液,適用于大多數(shù)的抗原
2.抗原熱修復(fù)時應(yīng)選擇的溫度。
據(jù)實驗認為溫度在92℃-98℃是合適的,尤其在95℃為,這是因為:(1)這種溫度未達沸騰,切片不容易脫離載玻片;(2)能夠解離和破壞與蛋白交聯(lián)的甲醛醛鍵等,處理時可以隨意選擇和確定抗原修復(fù)的作用時間。如果選擇高壓修復(fù),因為溫度較高,時間不宜過長
3.抗原修復(fù)時有效溫度所需持續(xù)時間根據(jù)各單位的設(shè)備條件以及使用的儀器不同,抗原修復(fù)時在有效的溫度范圍內(nèi)所持續(xù)的時間也不一樣。
4.抗原修復(fù)液必須遵循自然降溫規(guī)律,否則效果不好或達不到抗原修復(fù)的目的。
5.盡量使用足量的抗原修復(fù)液,防止切片干涸。
6.切片必須附貼牢固,否則發(fā)生掉片。
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相關(guān)標記抗體:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
β淀粉樣肽(1-42)抗體,Anti-beta Amyloid 1-42抗體簡單介紹:
產(chǎn)品別名:beta Amyloid(1-42); beta Amyloid 1-42; beta-Amyloid 1-42; Amyloid 1-42; P3(42); A4; AAA; ABETA; ABPP; AD1; Alzheimers Disease Amyloid Protein; Amyloid B; Amyloid Beta A4 Protein Precursor; Amyloid Beta; Amyloid of Aging and Alzheimer Disease; APP; APPI; B Amyloid; Beta APP; Cerebral Vascular Amyloid Peptide; CTFgamma; CVAP; PN II; PN2; PreA4; Protease nexin II; A beta; A4_HUMAN; Beta-amyloid protein 42.
抗體來源:Rabbit or Mouse
保質(zhì)期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:The cerebral and vascular plaques associated with Alzheimer's disease are mainly composed of Amyloid beta peptides. beta Amyloid is derived from cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides result from cleavage of Amyloid precursor protein after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last Amyloid precursor protein processing step. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides are major constituents of the plaques and tangles that occur in Alzheimer's disease. beta Amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimer's disease.
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