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簡(jiǎn)要描述:CCM-300葉綠素含量測(cè)量?jī)x是使用葉綠素?zé)晒獗嚷蕦?duì)葉綠素含量測(cè)量的一種新型儀器。與傳統(tǒng)的化學(xué)測(cè)量方法和吸收性測(cè)量方法相比,它可以對(duì)非常小的葉片以及難以測(cè)量樣品的葉綠素含量進(jìn)行非破壞性測(cè)量,測(cè)量迅速可靠。

詳細(xì)介紹

    一用途

    葉綠素含量測(cè)量?jī)x廣泛應(yīng)用于植物生理生態(tài)研究,特別是對(duì)傳統(tǒng)吸收法測(cè)量困難的小葉片或樣品的葉綠素含量進(jìn)行測(cè)量,例如針葉、發(fā)育未*的水稻、生于巖石上的絲狀藻、地衣、草坪草、仙人掌、龍舌蘭屬植物、菠蘿、擬南芥、果實(shí)、苔蘚、葉莖、葉柄。

 

 

    二原理

    盡管實(shí)踐已經(jīng)證明了吸收技術(shù)測(cè)量葉綠素含量的有效性,但該方法對(duì)樣品的要求較高,使其應(yīng)用范圍受到限制。而樣品本身的葉綠素?zé)晒鈩t不受樣品厚度、均一性等的影響,可以指示葉綠素的含量。根據(jù)這個(gè)原理,Gitelson等比較了葉片的吸收和反射對(duì)葉綠素?zé)晒獾挠绊懀l(fā)現(xiàn)在735nm和700nm處的比值與葉片的反射相關(guān)性,并且當(dāng)樣品葉綠素含量在41mg·m-2到675mg·m-2時(shí),F(xiàn)735/F700的比值與其葉綠素含量間存在著顯著的線性關(guān)系(R2≥0.95),與傳統(tǒng)的吸收性測(cè)量方法相比(測(cè)量范圍≤300mg·m-2),提高了葉綠素含量的線性關(guān)系范圍。

    根據(jù)Gitelson的研究結(jié)果,CCM-300使用具有15nm半峰寬的460nm二極管用于熒光激發(fā),提高了紅色熒光波長(zhǎng),避開(kāi)了大約685nm的發(fā)射熒光峰(葉綠素可重新吸收,降低測(cè)量精度)。儀器使用在735nm和700nm的葉綠素發(fā)射熒光的比率(F735/F700)進(jìn)行葉綠素含量的測(cè)量,讀數(shù)為F735/F700的比值以及根據(jù)Gitelson方程計(jì)算的葉綠素含量。

 

三 特點(diǎn)

 

Ø 直接算出葉綠素含量mg/m2;

 

Ø 測(cè)量結(jié)果可靠,不受葉片或樣品大小、厚度和形狀的影響;

 

Ø 經(jīng)濟(jì)、輕便、手持式設(shè)計(jì),適合野外使用;

 

Ø 與化學(xué)測(cè)量結(jié)果高度相關(guān),即使在高葉綠素含量時(shí);

 

Ø 彩色觸摸屏顯示;

 

Ø 離散的單點(diǎn)測(cè)量和多點(diǎn)(2-30個(gè)樣品)平均值測(cè)量模式;

 

Ø 非易失性閃存(2GB);

 

USB數(shù)據(jù)輸出,可使用Excel打開(kāi);

 

 

 

 

四 組成

 

主機(jī)、光纖樣品夾、電池充電器、4節(jié)AA鎳氫充電電池、USB通訊纜線、使用手冊(cè)。

 

可選配件:

 

20mm大探頭: 增加測(cè)量面積。 較大的樣本量可能對(duì)某些類型的樣本有用,例如苔蘚

 

 

 

 

 

 

 

增長(zhǎng)版葉夾:

 

 

 

 

 

 

 



技術(shù)參數(shù)

 


 

 

Ø 測(cè)量參數(shù):葉綠素?zé)晒獗嚷?/span>——發(fā)射熒光在735nm/700nm的比值(CFR),或相對(duì)葉綠素含量mg· m-2

 

Ø 測(cè)量面積:直徑3mm的圓,外徑4mm,但對(duì)直徑小于3mm的樣品也可準(zhǔn)確可靠的測(cè)量;

 

Ø 分辨率:比值0.011 mg· m-2;

 

Ø 重復(fù)性:依賴于信號(hào)強(qiáng)度,對(duì)信號(hào)強(qiáng)度低的樣品,建議使用多點(diǎn)測(cè)量的平均值,對(duì)信號(hào)強(qiáng)度高的樣品,差異通常為±0.03或更低;。

 

Ø 噪聲:<±0.02;

 

Ø 光源:LED 460 nm藍(lán)光二極管,半峰寬15 nm;

 

Ø 探頭:兩個(gè)固態(tài)高感傳感器,提供濾光片。同時(shí)提供兩個(gè)波長(zhǎng)探頭(700nm~710nm,730nm~740nm);

 

Ø 檢測(cè):數(shù)控調(diào)制光,將背景光影響將至低。并對(duì)光源和檢測(cè)器進(jìn)行溫度補(bǔ)償;

 

Ø 存儲(chǔ)容量:2GB;

 

Ø 模式:單點(diǎn)測(cè)量,2~30個(gè)點(diǎn)去平均值,去除2σ區(qū)間外的平均值,或者中位數(shù);

 

Ø 樣品接口:光纖傳感器,直徑4mm

 

Ø 用戶界面:240 × 320 像素彩色觸摸屏;

 

Ø 輸出:USB 1.1;

 

Ø 溫度范圍:0~50 ℃;

 

Ø 電源:2節(jié)充電AA電池;

 

Ø 自動(dòng)關(guān)閉間隔:(無(wú)按鍵或下載)0~20分鐘可選;

 

Ø 大?。?/span>12 cm × 9 cm × 3 cm;

 

Ø 重量0.16lbs 275g;

 

Ø 測(cè)量時(shí)間:5秒;

 


 

 

五、產(chǎn)地:美國(guó)

 

六、參考文獻(xiàn)

 

17天的水稻幼苗葉綠素含量的測(cè)定

 

Chia-Cheng Kan, Tsui-Yun Chung, Yan-An Juo Ming-Hsiun Hsieh (2015) Glutamine rapidly induces the expression of key transcription factor genes involved in nitrogen and stress responses in rice roots BMC Genomics201516:731 

 

2周大的擬南芥幼苗

 

Jasmina Kurepa, Timothy E. Shull, Jan A. Smalle (2016) Quercetin feeding protects plants against oxidative stress.

 

F1000Research 2016, 5:2430 Last updated: 25 DEC 2016) Latest published: 03 October 2016, 5:2430 (doi:

 

10.12688/f1000research.9659.1)

 

云杉樹(shù)苗

 

O Krišāns, ā Jansons, G Ievinsh (2016) Effect of decreased precipitation events on primary growth and photosynthesis-

 

related characteristics of spruce (Picea abies) saplings grown in different substrates - THE 74th SCIENTIFIC

 

CONFERENCE OF THE UNIVERSITY OF LATVIA   2016 - eeb.lu.lv

 

南極的苔蘚

 

Erin E. Shortlidge, Sarah M. Eppley, Hans Kohler, Todd N. Rosenstiel, Gustavo E. Zúñiga, Angélica

 

Casanova-Katny, (2017) Passive warming reduces stress and shifts reproductive effort in the Antarctic moss,

 

Polytrichastrum alpinum Annals of Botany, Volume 119, Issue 1, 1 January 2017, Pages 27–38,

 

 

 

 

珊瑚-波浪運(yùn)動(dòng)和營(yíng)養(yǎng)脅迫

 

Dana Riddle (2015) Coral Nutrition Part 4: Can Nutrient Deficiency Related to Water Motion Induce Coral

 

Blea

 

葉綠素

 

Shuai Liu, Su Li, Xiao-Yang Fan, Guo-Di Yuan, Tao Hu, Xian-Meng Shi, Jun-Biao Huang, Xiao-Yan Pu,

 

Chuan-Sheng Wu (2019) Comparison of two noninvasive methods for measuring the pigment content in foliose

 

macrolichens. et al. Photosynth Res (2019). 

 

果園草

 

Gordon B. Jones, Jasper B. Alpuerto, Benjamin F. Tracy, and Takeshi Fukao (2017) Physiological Effect of

 

Cutting Height and High Temperature on Regrowth Vigor in Orchard grass. Front Plant Sci. 2017; 8: 805.

 

Published online 2017 May 19. doi:  10.3389/fpls.2017.00805 PMCID: PMC5437204

 

7-42天的轉(zhuǎn)基因大麥

 

PetrVojta, FilipKokáš, Alexandra Husi?ková, Ji?íGrúz, VeroniqueBergougnoux, Cintia F. Marchetti, Eliška

 

Je?ilová, Václav Mik, Yoshihisa Ikeda, Petr Galuszka, (2016) Whole transcriptome analysis of transgenic

 

barley with altered cytokinin homeostasis and increased tolerance to drought stress. New Biotechnology

 

Volume 33, Issue 5, Part B, 25 September 2016, Pages 676-691 

 

蘇格蘭松樹(shù)幼苗

 

Tapani Repo, Samuli Launiainen, Tarja Lehto, Sirkka Sutinen, Hanna Ruhanen, Juha Heiskanen, Ari Laurén,

 

Raimo Silvennoinen, Elina Vapaavuori, Leena Finér (2016) The responses of Scots pine seedlings to

 

waterlogging during the growing season   Canadian Journal of Forest Research 2016, 46(12): 1439-1450,

 

 

 

 

挪威云杉和蘇格蘭松樹(shù)苗

 

Johanna Riikonen, Nelli Kettunen, Maria Gritsevich, Teemu Hakala, Liisa Särkkä, Risto Tahvonen, (2016)

 

Growth and development of Norway spruce and Scots pine seedlings under different light spectra

 

Environmental and Experimental Botany Volume 121, January 2016, Pages 112-

 

泥炭地植被泥炭蘚和其他泥炭地植物

 

Anshu Rastogi, Marcin Stró?eckia, Hazem M. Kalaji, Dominika ?uców de Mariusz, Lamentowicz, Rados?aw

 

Juszczak (2019) Impact of warming and reduced precipitation on photosynthetic and remote sensing properties

 

of peatland vegetation Environmental and Experimental Botany Volume 160, April 2019, Pages 71-80

 

CAM植物 - 仙人掌

 

Kudakwashe Musengi (2018) THE BIOLOGICAL CONTROL OF CACTI (CACTACEAE: OPUNTIOIDEAE)

 

IN SOUTH AFRICA: BASIS OF HOST SELECTION IN THE 'STRICTA' BIOTYPE OF

 

DACTYLOPIUS OPUNTIAE (COCKERELL) (HEMIPTERA: DACTYLOPIIDAE). University of the

 

Witwatersrand, South Africa. May 2018.  

 

高爾夫綠草和銀線苔蘚

 

ZRaudenbush Z.T, Greenwood J.L., McLetchie D.N., Eppley D.N. (2018) Divergence in Life-History and

 

Developmental Traits in Silvery-Thread Moss (Bryum argenteum Hedw.) Genotypes between Golf Course

 

Putting Greens and Native Habitats. Cambridge University Press: 11 September 2018, Volume 66, Issue 5 ,

 

September 2018 , pp. 642-650 , DOI:

 

苗圃氮素狀況

 

MJ Clark, Y Zheng (2017) Effect of Top-dressed Controlled-release Fertilizer Rates on Nursery Crop

 

Quality and Growth and Growing Substrate Nutrient Status in the Niagara Region, Ontario, Canada doi:

 

10.21273/HORTSCI11309-16 HortScience January 2017 vol. 52 no. 1 167-173

 

大豆

 

Pradeep Wagle, Prasanna H. Gowda, Saseendran S. Anapalli, Krishna N. Reddy, Brian K. Northup, (2017)

 

Growing season variability in carbon dioxide exchange of irrigated and rainfed soybean in the southern United

 

States. Science of The Total Environment,–Volumes 593594, 1 September 2017, Pages 263-273

 

 

 

 

土豆

 

Maropeng Vellry Nemutanzhela, David Mxolisi Modise, Kotose Joseph Siyoko, Sheku Alfred Kanu (2017)

 

Assessment of Growth, Tuber Elemental Composition, Stomatal Conductance and Chlorophyll Content of Two

 

Potato C*rs Under Irrigation with Fly Ash-Treated Acid Mine Drainage. American Journal of Potato

 

Researc August 2017, Volume 94,  Issue4, pp 367–378

 

十字花科-開(kāi)花植物

 

Soledad Martos, Berta Gallego, Llorenç Sáez, Javier López-Alvarado, Catalina Cabot, and Charlotte

 

Poschenrieder (2016) Characterization of Zinc and Cadmium Hyperaccumulation in Three Noccaea

 

(Brassicaceae) Populations from Non-metalliferous Sites in the Eastern Pyrenees Front Plant Sci. 2016; 7: 128.

 

Published online 2016 Feb 9. doi:10.3389/fpls.2016.00128

 

毒藤

 

John G. Jelesko, Elise B. Benhase, Jacob N. Barney (2017) Differential Responses to Light and Nutrient

 

Availability by Geographically Isolated Poison Ivy Accessions. Northeastern Naturalist 24(2):191-200. 2017

 

 

 

 

糖楓

 

Amritpal S. Singh, A. Maxwell P. Jones, Mukund R. Shukla, Praveen K. Saxena (2017) High light intensity

 

stress as the limiting factor in micropropagation of sugar maple (Acer saccharum Marsh). Plant Cell, Tissue and

 

Organ Culture (PCTOC), May 2017, Volume 129,  Issue2, pp209–221, doi:10.1007/s11240-017-1170-2

 

道路旁植物中的鎘和銅脅迫

 

Margita Kuklová, Helena Hnili?ková, František Hnili?ka, Ivica Pivková, Ján Kukla (2019) Impact of

 

expressway on physiology of plants and accumulation of risk elements in forest ecosystems Plant Soil Environ.,

65: 46 53. 


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