Thermo Scientific Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to UltraLink Biosupport. UltraLink Biosupport is a rigid, hydrophilic, highly crosslinked, copolymeric and porous resin with high coupling capacity. The porosity, rigidity, and durability of the resin make it suitable for medium-pressure, fast-flow techniques involving large sample volumes.
For protein purification or IP, resin is added to a sample containing DYKDDDDK-tagged proteins with the tag on either the N- or the C-terminus. Following capture of DYKDDDDK-tagged proteins, non-specifically bound proteins can be washed away before dissociating bound target proteins with elution buffer. The Pierce Anti-DYKDDDDK Affinity Resin is recommended for use in spin purification columns or cartridges for FPLC instruments.
Features include:
• Specific—unique base beads and highly specific antibody minimize off-target binding (low non-specific binding)
• High purity—optimized bind-wash-elute protocol enables high purity
• High yield—special antibody conjugation method enables high yield
• Rapid—entire purification protocol typically takes less than 40 mins
• Economical—purification protocol allows multiple reuses
• Versatile—can be used in spin-, gravity-, or FPLC-based purification and enrichment methods
• Scalable—available in 1, 5, and 50 mL settled resin package sizes to allow for smaller scale immunoprecipitations or larger scale purifications
Characteristics of Pierce Anti-DYKDDDDK Affinity Resin:
Composition: anti-DYKDDDDK antibody covalently attached to UltraLink Biosupport, a highly crosslinked rigid support
Particle size: 50–80 µm
pH stability: 1–13
Recommended linear velocity: <150 cm/hour
Operating temperature: 2–30?C; do not freeze
Particle concentration:50% slurry in phosphate buffered saline, 0.02% sodium azide, pH 7.5
Binding capacity: ≥3.0 mg DYKDDDDK-tGFP-His protein (∼32 kDa)/mL settled resin
For Research Use Only. Not for use in diagnostic procedures.
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