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CCL-13 Chang liver 人張氏肝細(xì)胞

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產(chǎn)品型號CCL-13 Chang liver

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CCL-13 Chang liver 人張氏肝細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻和*培養(yǎng)條件！

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CCL-13 Chang liver 人張氏肝細(xì)胞 的詳細(xì)介紹

CCL-13 Chang liver 人張氏肝細(xì)胞

ATCC^® Number:

CCL-13™

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$323.00

Additional information about this cell line
Designations:	HeLa [Chang Liver]
Depositors:	RS Chang
Biosafety Level:	2 [Cells contain human papilloma virus （HPV-18） ]
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens （human）
Morphology:	epithelial
Source:	Organ: HeLa contaminant
Cellular Products:	keratin
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:	transfection host （Roche FuGENE® Transfection Reagents）
Virus Susceptibility:	poliovirus 1, 2, 3; adenovirus 3; vesicular stomatitis （Indiana）
Tumorigenic:	Yes
Reverse Transcript:	negative
DNA Profile （STR）:	Amelogenin: X CSF1PO: 10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18
Isoenzymes:	G6PD, A
HeLa Markers:	Y
Comments:	This line was originally thought to be derived from normal liver tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining. NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
Propagation:	ATCC complete growth medium: Minimum essential medium （Eagle） in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10% Temperature: 37.0°C
Subculturing:	Protocol: Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow flask to sit at room temperature （or at 37C） until cells detach （approximay 10 minutes）. Add fresh medium, aspirate and dispense into new flasks. *Subction Ratio:** A subction ratio of 1:4 to 1:8 is recommended Medium Renewal:* 2 to 3 times per week
Preservation:	Complete growth medium, 95%; DMSO, 5%
References:	22213: Chang RS. Continuous subc*tion of epithelial-like cells from normal human tissues. Proc. Soc. Exp. Biol. Med. 87: 440-443, 1954. PubMed: 13237268 26122: Murphy WH, Landau BJ. Clonal variation and interaction of cells with viruses. Natl. Cancer Inst. Monogr. 7: 249-271, 1962. PubMed: 14477443 32352: Benn J, et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004

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iec-6 大鼠小腸隱窩上皮細(xì)胞
馬-達二氏犬腎細(xì)胞系 MDCK （NBL-2） CRL-34
鴨子胚胎成纖維細(xì)胞　 Duck embryo　 CCL-141
小鼠胚胎成纖維細(xì)胞 3T3-L1 CCL-173
人結(jié)腸直腸腺癌 COLO 320DM CCL-220
人結(jié)腸直腸腺癌 NCI-H508 CRL-253
人結(jié)腸直腸腺癌 COLO 320HSR CCL-220.1
人B淋巴細(xì)胞 Ramos（RA1） CRL-1596
人淋巴母細(xì)胞 T2（174×CEM.T2） CRL-1992
大鼠心肌細(xì)胞 H9C2（2-1） CRL-1446
豬鼻甲黏膜成纖維細(xì)胞 PT-K75 CRL-2528
小鼠胰腺癌細(xì)胞 LTPA CRL-2389
大鼠胰腺癌細(xì)胞 DSL-6A/C1 CRL-2132
小鼠骨髓瘤B淋巴細(xì)胞 SP2/MIL-6 CRL-2016
大鼠腦間質(zhì)細(xì)胞 DI TNC1 CRL-2005
小鼠肌原細(xì)胞 C2C12 CRL-1772
鵪鶉?yán)w維肉瘤細(xì)胞 QT6 CRL-1708
大鼠回腸細(xì)胞 IEC-18 CRL-1589

CCL-13 Chang liver 人張氏肝細(xì)胞
非洲綠猴腎細(xì)胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586
人成纖維細(xì)胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮細(xì)胞 WPE-int CRL-2888
人正常前列腺成肌纖維細(xì)胞 WPMY-1 CRL-2854
人胰腺導(dǎo)管腺癌細(xì)胞 PL45 CRL-2558
人胰腺癌細(xì)胞 Panc 05.04 CRL-2557
人胰腺癌細(xì)胞 Panc 04.03 CRL-2555
人胰腺癌細(xì)胞 Panc 02.03 CRL-2553
人胰腺癌細(xì)胞 Panc 08.13 CRL-2551
人胰腺癌細(xì)胞 Panc 03.27 CRL-2549
人胰腺癌細(xì)胞 Panc 10.05 CRL-2547
人肝癌細(xì)胞 SNU-398 CRL-2233
人成神經(jīng)瘤細(xì)胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌細(xì)胞 HPAC CRL-2119
人胰腺癌細(xì)胞 HPAF-II CRL-1997
人黑色素瘤細(xì)胞 A375.S2 CRL-1872
人臍靜脈內(nèi)皮細(xì)胞 HUV-EC-C CRL-1730
人黑素瘤細(xì)胞 WM-266-4 CRL-1676
人膀胱癌細(xì)胞 HT-1376 CRL-1472
人正常前列腺上皮細(xì)胞 RWPE-2 CRL-11610
人正常前列腺上皮細(xì)胞 RWPE-1 CRL-11609
雞胚胎成纖維細(xì)胞 UMNSAH/DF-1 CRL-12203
人乳腺癌細(xì)胞 MDA-MB-231 HTB-26
小鼠巨噬細(xì)胞 RAW 264.7 TIB-71
人橫紋肌肉瘤細(xì)胞 A-204 HTB-82

人胰腺癌細(xì)胞 Capan-2 HTB-80
人胰腺癌細(xì)胞 Capan-1 HTB-79
人乳腺導(dǎo)管癌細(xì)胞 MDA-MB-435S HTB-129
人胃癌細(xì)胞 Hs 746T HTB-135
人肺腺癌細(xì)胞 NCI-H441 [H441] HTB-174
人骨髓瘤細(xì)胞 U266 TIB-196
人結(jié)腸癌細(xì)胞 NCI-H716 [H716] CCL-251
人多形性惡性膠質(zhì)瘤 T98G [T98-G] CRL-1690
人神經(jīng)母細(xì)胞瘤細(xì)胞 BE（2）-C CRL-2268
人腎透明細(xì)胞癌細(xì)胞 Caki-2 HTB-47
人皮膚成纖維細(xì)胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤細(xì)胞系
CRL-5826 NCI-H226 人肺鱗癌細(xì)胞系
Tca-8113 人舌鱗癌細(xì)胞
MA-891 小鼠乳腺癌高轉(zhuǎn)移細(xì)胞
U14 小鼠宮頸癌細(xì)胞
CRL-9518 L5178Y 小鼠淋巴瘤細(xì)胞
HTB-112 HEC-1-A 人子宮內(nèi)膜腺癌細(xì)胞
HTB-113 HEC-1-B 人子宮內(nèi)膜腺癌細(xì)胞
AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞
ROS1728 大鼠成骨細(xì)胞
CRL-6323 B16F1 小鼠黑色素瘤細(xì)胞
HTB-58 SK-MES-1 人肺鱗癌細(xì)胞
CCL-127 IMR-32 人神經(jīng)母細(xì)胞瘤細(xì)胞
HTB-79 Capan-2 人胰腺癌細(xì)胞

BALL-1 人B淋巴細(xì)胞急性白血病細(xì)胞
CRL-2922 Eayh926 人臍靜脈細(xì)胞融合細(xì)胞
WB-F344 大鼠肝干細(xì)胞
CRL-1715 TM4 正常小鼠睪丸細(xì)胞
CRL-2539 4T1 小鼠乳腺癌細(xì)胞
CRL-1442 BRL-3A 大鼠肝細(xì)胞
BRL 大鼠肝細(xì)胞
RSC96大鼠雪旺細(xì)胞
Isk 人子宮內(nèi)膜癌細(xì)胞
HTB-113 hec-1-b 人子宮內(nèi)膜腺癌細(xì)胞
PLA-802?人惡性橫紋肌肉瘤
Pt-K2 袋鼠腎細(xì)胞
BS-C-1 非洲綠猴腎細(xì)胞
A498人腎癌細(xì)胞
3AO人卵巢癌細(xì)胞
iec-6 大鼠小腸隱窩上皮細(xì)胞
u87MG 人腦星形膠質(zhì)母細(xì)胞瘤
Renca 小鼠腎癌細(xì)胞
nci-h929 骨髓瘤細(xì)胞
P388小鼠白血病細(xì)胞
opm-2 骨髓瘤細(xì)胞
MDA-MB-435 人乳腺癌高轉(zhuǎn)移細(xì)胞
KU812 人外周血嗜堿性白細(xì)胞

NIH:OVCAR-3 人卵巢癌細(xì)胞
SW 1990人胰腺癌細(xì)胞系
S180人結(jié)腸腺癌細(xì)胞
HuTu-80十二指腸腺癌
RL95-2 人子宮內(nèi)腺癌細(xì)胞
NCI-H446 人小細(xì)胞肺癌細(xì)胞
MSTO-211H 人肺癌細(xì)胞
H4-Ⅱe 大鼠肝癌細(xì)胞系
HLF 人肺成纖維樣細(xì)胞
LS 180人結(jié)腸腺癌細(xì)胞
HuTu-80十一指腸腺癌
ST豬睪丸細(xì)胞系
REH人急性非B非T淋巴細(xì)胞白血病
NCI-H520 人肺鱗癌細(xì)胞
RKO 人結(jié)腸腺癌細(xì)胞
293A 人胚腎細(xì)胞
RK-13 兔腎細(xì)胞系