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CCL-186 IMR-90 人胚肺成纖維細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種，細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件！

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CCL-186 IMR-90 人胚肺成纖維細(xì)胞 的詳細(xì)介紹

CCL-186 IMR-90 人胚肺成纖維細(xì)胞
ATCC® Number: CCL-186™
Designations: IMR-90
Depositors: WW Nichols
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens （human）
Morphology: fibroblast

Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host （Nucleofection technology from Lonza）
Virus Susceptibility: Human poliovirus 1
Human poliovirus 2
Varicella-Zoster
Herpes simplex virus 1
Herpes simplex virus 2
Human poliovirus 3
Vaccinia virus
Human herpesvirus 5
Vesicular stomatitis virus
Reverse Transcript: negative
DNA Profile （STR）: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,19
Cytogenetic Analysis: normal human female; diploid; stable
Isoenzymes: G6PD, B
Age: 16 weeks gestation
Gender: female
Ethnicity: Caucasian
Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381]
The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.
The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339
32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642
33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564
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CCL-186 IMR-90 人胚肺成纖維細(xì)胞
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人正常前列腺成肌纖維細(xì)胞 WPMY-1 CRL-2854
人胰腺導(dǎo)管腺癌細(xì)胞 PL45 CRL-2558
人胰腺癌細(xì)胞 Panc 05.04 CRL-2557
人胰腺癌細(xì)胞 Panc 04.03 CRL-2555
人胰腺癌細(xì)胞 Panc 02.03 CRL-2553
人胰腺癌細(xì)胞 Panc 08.13 CRL-2551
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人結(jié)腸癌細(xì)胞 NCI-H716 [H716] CCL-251
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人神經(jīng)母細(xì)胞瘤細(xì)胞 BE（2）-C CRL-2268
人腎透明細(xì)胞癌細(xì)胞 Caki-2 HTB-47
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BALL-1 人B淋巴細(xì)胞急性白血病細(xì)胞

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WB-F344 大鼠肝干細(xì)胞
CRL-1715 TM4 正常小鼠睪丸細(xì)胞
CRL-2539 4T1 小鼠乳腺癌細(xì)胞
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BRL 大鼠肝細(xì)胞
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HuTu-80十一指腸腺癌
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REH人急性非B非T淋巴細(xì)胞白血病
NCI-H520 人肺鱗癌細(xì)胞
RKO 人結(jié)腸腺癌細(xì)胞
293A 人胚腎細(xì)胞
RK-13 兔腎細(xì)胞系