抗腦抗體(ABAb)檢測試劑盒Human anti-brain tissue antibody,ABAb ELISA kit齊一生物科技(上海)有限公司以真誠、主動、周到、規(guī)范、熱情的服務(wù)很快得到廣大客戶的*好評.公司秉承“適應(yīng)多樣化的需求,提供專業(yè)化的服務(wù)"的經(jīng)營理念,為客戶提供*的服務(wù)
抗腦抗體(ABAb)檢測試劑盒操作步驟
實(shí)驗(yàn)開始前,請?zhí)崆芭渲煤盟性噭?,試劑或樣品稀釋時,均需混勻,混勻時盡量避免起泡。每次檢測都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過高時,用樣品稀釋液進(jìn)行稀釋,以使樣品符合試劑盒的檢測范圍。
1. 加樣:分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測樣品孔。空白孔加樣品稀釋液100μl,余孔分別加標(biāo)準(zhǔn)品或待測樣品100μl,注意不要有氣泡,加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻,酶標(biāo)板加上蓋或覆膜,37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性,每次實(shí)驗(yàn)請使用新的標(biāo)準(zhǔn)品溶液。
2. 棄去液體,甩干,不用洗滌。每孔加*標(biāo)記抗體工作液 100μl(取1μl*標(biāo)記抗體加99μl*標(biāo)記抗體稀釋液的比例配制,輕輕混勻,在使用前一小時內(nèi)配制),37℃,60分鐘。
3. 溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板3次,每次浸泡1-2分鐘,350μl/每孔,甩干。
4. 每孔加辣根過氧化物酶標(biāo)記親和素工作液(同*標(biāo)記抗體工作液) 100μl,37℃,60分鐘。
5. 溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板5次,每次浸泡1-2分鐘,350μl/每孔,甩干。
6. 依序每孔加底物溶液90μl,37℃避光顯色(30分鐘內(nèi),此時肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色,后3-4孔梯度不明顯,即可終止)。
7. 依序每孔加終止溶液50μl,終止反應(yīng)(此時藍(lán)色立轉(zhuǎn)黃色)。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性,底物反應(yīng)時間到后應(yīng)盡快加入終止液。
8. 用酶聯(lián)儀在450nm波長依序測量各孔的光密度(OD值)。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測。
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9019404 Board, distribution, no PTCs, RG3
9019389 LED, blue (470), RG3
9019371 Hinge assembly, front hinge plate, RG6
9019358 Transformer, low volts, RG6
1027294 AllStars Neg. siRNA AF 555 (20 nmol)
9019343 LED, HRM, RG6
9019326 Belt, toothed, src, detector optics, RG6
9234807 Trough Lid Digene
9234677 Board, shaker, non-CE
9234466 Clamp, rubber, adapter plate, HS shaker,
9233455 Ring, straining, pressure sensor
9233443 Valve, dry way, auto vac, BR8000
9019233 Valve - Dispense, PyroQ24
9019218 Lead screw - Z robot, PyroQ96MD
9019213 PCB 6 - Connection, PyroQ96MD
9019187 Camera MX9, PyroQ96MD
1020953 Buffer AVE + Azid 108 x (2/2),KG
1061096 HSV Quantification Standards
1061119 HBV Controls & Quantification Standards
9016743 Software Pack. LiquiChip Policy Manager
9019867 Transformer (230V), HS
9019865 Riser block, 19.5 mm, QXT
9019760 Power supply, high voltage, V2, QX
9019730 Board, smart key, V2, QX
9013674 Upgrade-Kit Shakerplatte autom BR8000
9013610 Reagent holder, 5-trough 80 ml
9013428 Maintenance kit, semi annual, BR9600 (B)
9013423 Tubing set, with HSPS, BR9600
9019666 Set Adjustment plate, Qsym
9019640 Temperature Controller, EZ1xl
9019631 Board, main assembly, HS
9019601 O-ring, filter housing seal 2.5mm, CX1
9019586 Power supply, QGX
9019583 Slideout electrical tray (220V), C1x
9019429 Sensor, detector home, RG3
9019364 Motor, stepper no cable, detector, RG6
9019363 Motor-stepper, rotordrive,sourceopt, RG6
1027416 FlexiTube GeneSolution siRNA 1 nmol
9019337 Photomultiplier with cable, RG6
9234679 Rack for Murex Reagents VK54/55,VK
9020473 Optic fibre, 550 mm, GSC
9234552 Connector, tubing, 1/8 in to 6 mm tubing
1027281 AllStars Neg. Control siRNA (20 nmol)
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1026577 DyeEx 2.0 Spin Kit (10)
9234262 Frame, cooling, BR3000, 0
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1062737 FastLane Cell cDNA Buffer Set (14x250ml)
9232909 Board, Z2 motor
9232907 Board, option, non-CE
1061052 HBV Quantification Standards
1061067 Influenza A RT-PCR REAGENT
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9016537 Transportation box, BioSprint 96
9016463 Vacuum Tray, complete, BR8 RD, UK
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