現(xiàn)貨供應(yīng)S100B蛋白(S-100B)檢測(cè)試劑盒英文Human Soluble protein-100B,S-100B ELISA Kit齊一生物針對(duì)中客戶推出自主品牌 ELISA KIT(96T/48T),為了保證客戶用貨質(zhì)量,我公司采用純進(jìn)口原料現(xiàn)包被生產(chǎn),質(zhì)量穩(wěn)定,性價(jià)比高!!提供代測(cè)服務(wù)!全國:
現(xiàn)貨供應(yīng)S100B蛋白(S-100B)檢測(cè)試劑盒一、高效、靈敏、特異的抗體;
二、穩(wěn)定的重復(fù)性和可靠性;
三、吸附性能好,空白值低,孔底透明度高的固相載體;
四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型;
五、節(jié)省實(shí)驗(yàn)經(jīng)費(fèi).
elisa試劑盒回收率是反應(yīng)待測(cè)物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說你的回收率是99%,這個(gè)與真實(shí)成分有密切的關(guān)系,說明方法的準(zhǔn)確度.比如水中總無機(jī)氯含量測(cè)定,樣品水中含有無機(jī)氯20mg/L,取100mL被測(cè)水樣品,加入0.1mL濃度為10mg/mL的含無機(jī)氯標(biāo)準(zhǔn)樣品,測(cè)定時(shí)忽略體積變化,如果測(cè)定出樣品中無機(jī)氯為2.98mg/L,則認(rèn)為回收率為99%.
制備方法
包括以下步驟:
1)抗原表位的計(jì)算機(jī)篩選;
2)抗原的制備;
3)血清的采集;
4)間接ELISA方法的建立;
5)間接ELISA方法的敏感性和特異性驗(yàn)證;
6)試劑盒的穩(wěn)定性驗(yàn)證;
7)對(duì)屠宰場進(jìn)行臨床檢測(cè).該方法簡單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出感染豬的戊型肝炎病毒,適用于大批量發(fā)病樣本的檢測(cè),可用于豬戊肝的快速診斷,并預(yù)防、控制豬戊肝病毒的流行和阻斷戊肝病毒由豬向人傳播.
組成結(jié)構(gòu)
1、血清:操作過程中避免任何細(xì)胞刺激.使用不含熱原和內(nèi)毒素的試管.收集血液后,1000×g離心10分鐘將血紅細(xì)胞迅速小心地分離.
2、血漿:EDTA、檸檬酸鹽、肝素血漿可用于檢測(cè).1000×g離心30分鐘去除顆粒.
3、細(xì)胞上清液:1000×g離心10分鐘去除顆粒和聚合物.
4、組織勻漿:將組織加入適量生理鹽水搗碎.1000×g離心10分鐘,取上清液.
5、保存:如果樣品不立即使用,應(yīng)將其分成小部分-70℃保存,避免反復(fù)冷凍.盡可能的不要使用溶血或高血脂血.如果血清中大量顆粒,檢測(cè)前先離心或過濾.不要在37℃或更高的溫度加熱解凍.應(yīng)在室溫下解凍并確保樣品均勻地充分解凍.
9019577 Opto switch, Z-axis, 1 ch. , Cx2
9019594 Belt, Z-axis gearbox (black), 8 ch., Cx8
9019068 PyroMark CpG SW 1.0 (5) "Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies.
Five additional software licenses for PyroMark CpG SW 1.0"
9019086 PyroMark Q96 MD Software (5) "For use with PyroMark Q96 MD instrument.Software for setting up a run and analyzing mutations.
5 licenses"
9019658 O rings - VPT Fast connector, PyroVW
972012 PyroMark Q96 CpG p16 (96) For quantification of methylation level in p16
979003 PyroMark Q96 Sample Prep Tool Cover (10) Consumables for use with magnetic tool for isolation of single stranded DNA.
979008 PyroMark Wash Buffer (conc., 200 ml) For washing and neutralizing the immobilized DNA.
950140 Binding Additive (DX) (4.0 g)
9019803 Upgrade Package 1, RCS
130803 NeXtal CubicPhase 1w Mo μplate (10)
9243044 Set, brackets, transparent panel, Qsym
283615 virotype PEDV/TGEV RT-PCR Rgt (96)
52906 QIAamp Viral RNA Mini Kit (250) For 250 RNA preps: 250 QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), RNase-free Buffers
63183 DyeEx 96 Kit (24) 24 DyeEx 96 Plates; 4 Collection Plates, 48-Well
69582 DNeasy 96 Blood & Tissue Kit (12) For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers
70042 Oligotex mRNA Midi Kit For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers
79523 Small Spin Columns 24 RNase-free spin columns for Oligotex spin procedures
301109 PolyFect Transfection Reagent (10x100ml)
9239361 PCB-micro-fuse T 6.3A, 250V, EZ1
132632 NeXtal Stock Potassium formate (50) 10M Potassium formate - 50mL (4 x 12,5 ml tube)
9019610 Cover, Pipettor, 1 ch. (white), CAS
9019624 PCB, lge breakout, 12 core to rev 3, Cx2
9019067 PyroMark CpG SW 1.0 Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies.
9019087 PyroMark IdentiFire SW 1.0 Software for sequence-based species identification by comparing and matching Pyrosequencing data to a local database.
972052 PyroMark Q96 CpG Prader Willi (96) For quantification of methylation level in Prader-Willi and Angelman Syndromes
979002 PyroMark Q96 Plate Low (100) For sample analysis of DNA template on ID.
979004 PyroMark Q96 Cartridge (3) For delivery of nucleotides and reagents for use in PyroMark Q96 ID.
9001513 PyroMark Q24 MDx For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
990610 Filter-Tips, OnCor C, 200 μl (960)
9017842 Tool, gauge, 30 mm, calibration, QIAC
9020806 Rotor Timing Calibration Dye, RGQ
331455 miScript Microfluidics PreAMP Kit (96)
381566 Investigator ESSplex SE GO! Kit (200)
9022654 Transfer Carriage QIAplate, QXT
16191 QIAwell 96 Ultra Plasmid Kit (4) For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, and QIAprep 96 Plates; Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
19046 Plasmid Buffer Set Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps
19201 Collection Tubes (2-ml) 1000 Collection Tubes (2 ml)
9015862 EZ1 DNA PRFN Card Pre-programmed card for BioRobot EZ1 paraffin section protocols
67163 MagAttract 96 DNA Plant Core Kit (24) MagAttract Suspension A and buffers for 24 x 96 preps
1030483 Elution Microtubes CL, PAX, FLU
9232687 Hood f. Tip Disposal Station 2.01
9015972 Tube Holder, STS MDx, 15 mm (12)
158926 Cell Suspension Solution (125 ml)
159992 Generation DNA Purif. Soln 1 (1000 ml)
201913 dNTP Set, PCR Grade 100mM, (4x250μl) dNTP Set is a complete set of individual, highly pure dNTPs: 100 mM each dATP, dCTP, dGTP, dTTP for 2500 x 50ul PCR reactions
12945 QIAGEN Plasmid Plus Midi Kit (100) "100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges
QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega and Giga) or up to 24 (Midi and Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."
9019715 PyroMark Q24 User Manual
9019861 Rotor Locking Nut Set
9019890 Foam insert, rotor securing, QIAC
336391 SureSilencing shRNA Control
330522 RT2 SYBR Green ROX qPCR Mastermix (12)
330411 RT2 HT First Strand Kit (96)
04814340 Acrylamide Electrophoresis Grade 丙烯酰胺 電泳級(jí)別 100 g
04814343 Acrylamide Electrophoresis Grade 丙烯酰胺 電泳級(jí)別 250 g
04814346 Acrylamide Electrophoresis Grade 丙烯酰胺 電泳級(jí)別 500 g
04814349 Acrylamide Electrophoresis Grade 丙烯酰胺 電泳級(jí)別 1 kg
04814320 Acrylamide, Ultra Pure 丙烯酰胺 超純 100 g
04814323 Acrylamide, Ultra Pure 丙烯酰胺 超純 250 g
04814326 Acrylamide, Ultra Pure 丙烯酰胺 超純 500 g
04814329 Acrylamide, Ultra Pure 丙烯酰胺 超純 1 kg
11BIAC1902 Acrylamide-Bis-Acryl 19:1 丙烯酰胺-甲叉雙丙烯酰胺 19:1 1 L
11BIAC2902 Acrylamide-Bis-Acryl 19:1 丙烯酰胺-甲叉雙丙烯酰胺 29:1 1 L
11BIAC3752 Acrylamide-Bis-Acryl 37,5:1 丙烯酰胺-甲叉雙丙烯酰胺 37.5:1 1 L
04800802 LIQUI-GEL? 19:1 (40% Acrylamide Bis - Acrylamide Solution) LIQUI-GEL? 19:1(40%丙烯酰胺-甲叉雙丙烯酰胺溶液) 500 ml
04800803 LIQUI-GEL? 29:1 (40% Acrylamide Bis - Acrylamide Solution) LIQUI-GEL? 29:1(40%丙烯酰胺-甲叉雙丙烯酰胺溶液) 500 ml
04800804 LIQUI-GEL? 37.5:1 (40% Acrylamide Bis - Acrylamide Solution) LIQUI-GEL? 37.5:1(40%丙烯酰胺-甲叉雙丙烯酰胺溶液) 500 ml
緩沖液
DTA Disodium Salt, Dihydrate, Molecular Biology reagen EDTA二鈉鹽 500 g
11EDTA05M1 EDTA EASY SOL EDTA二鈉鹽溶液 100 ml
112350200 ETBR Green Bag Disposal Kit 溴化乙錠去除試劑盒 50 bags
04802511 Ethidium Bromide, Solution (10mg/ml) 溴化乙錠溶液 10 ml
11TAE50X01 TAE 50X Buffer TAE 50X 緩沖液 1 L
11TBE10X02 TBE 10X Buffer TBE 10X 緩沖液 1 L
04816116 Tris HCl, Ultra Pure TRIS HCL,超純 100 g
Tris, Molecular Biology Reagent TRIS,分子生物學(xué)級(jí)別 1 kg
培養(yǎng)基
Casein, Vitamin Tested 30 Mesh 酪蛋白 1 lb
Casein, Vitamin Tested 30 Mesh 酪蛋白 5 lb
Casein, Vitamin Tested 30 Mesh 酪蛋白 25 lb
Casein, Vitamin Tested 30 Mesh 酪蛋白 100 lb
Agar (Bacteriological Grade) 瓊脂(細(xì)菌學(xué)級(jí)別) 100 g
Agar (Bacteriological Grade) 瓊脂(細(xì)菌學(xué)級(jí)別) 250 g
Agar (Bacteriological Grade) 瓊脂(細(xì)菌學(xué)級(jí)別) 500 g
Casein Hydrolysate 酪蛋白水解物 1 lb
Casein Hydrolysate 酪蛋白水解物 5 lb
Casein Hydrolysate 酪蛋白水解物 25 lb
Zein 玉米醇蛋白 100 g
Zein 玉米醇蛋白 500 g
Zein 玉米醇蛋白 1 kg